溶解氧对OLAND生物膜反应器硝化性能的影响及其微生物种群动态研究  

The influence of DO on nitrification performance and microbial community changes of biofilm in OLAND system

作　　者：董远湘[1] 李小明[1] 尹疆[1] 杨麒[1] 钟琼 

机构地区：[1]湖南大学环境科学与工程系,湖南长沙410082 [2]湖南环境保护研究所,湖南长沙410082

出　　处：《环境污染与防治》2005年第8期561-564,共4页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.50748054);国际科技合作重点项目(No.2003AA649020)

摘　　要：采用以多孔球悬浮填料为载体的限氧亚硝化生物膜处理高氨氮、低碳源的废水,通过对DO控制在0.5～1.0 mg/L,实现硝化阶段出水中的氨氮与亚硝态氮的比例达到最适值1:(1.2±0.2),从而为后阶段的厌氧氨氧化系统提供理想的进水,进而提高氮的去除率;同时应用PCR-DGGE对硝化阶段不同时期的生物膜中微生物的种群动态变化进行了分析.研究表明,群落结构和优势种群的数量具有时序动态性,微生物多样性与废水的处理效果出现协同变化的特征.测序结果表明,在生物膜中进行氨氧化作用的主要为亚硝化杆菌(Nitrosomonas sp.)、亚硝化螺菌(Nitrosospira sp.);进行亚硝酸氧化的主要为硝化球菌(Nitrococcus sp.).The biofilm with porous global suspended fillers from oxygen-limited autotrophic nitrification-denitrification （OLAND） system treated high ammonium concentration and a low chemical oxygen demand. A steady ratio of ammonium and nitrite[1：（1.2±0.2）1 form the effluent in the first stage （nitritation stage）was attained by controlling the dissolved oxygen in the range of 0.5±1.0 mg/L,which supplied more perfect influent for the stage of anaerobic ammonium oxidation. Performance of nitritation stage was focused in this study, and denaturing gradient gel electrophoresis（DGGE） was applied to analyze the shift of microbial community in different stage. The profile of DC, GE show that different biofilm had the different bands＇ patterns. In the profile show that dynamics of community structure and amount of dominant populations were responsible to different period. The sequences revealed that Nitrosornonas sp. and Nitrosospira sp. was the predominant species for ammonia oxidation; Nitrococcus sp. was the predominant species to oxidize nitrite.

关 键 词：变性梯度凝胶电泳 生物膜 微生物种群结构 硝化性能 

分 类 号：X703[环境科学与工程—环境工程] S476[农业科学—农业昆虫与害虫防治]