Function of c-Fos-like and c-Jun-like Proteins on Trichostatin A-induced G_2/M Arrest in Physarum polycephalum  

作　　者：Xiao-Xue LI Jun LU Yan-Mei ZHAO Bai-Qu HUANG 

机构地区：[1]Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第11期767-772,共6页生物化学与生物物理学报（英文版）

基　　金：The work was supported by the grants from the National Natural Science Foundation of China(30571698);the Major State Basic Research Project of China(2005CB522404);the Young Teacher Foundation of Northeast Normal University(111494025)

摘　　要：The homologs of transcription factors c-Fos and c-Jun have been detected in slime mold Physarum polycephalum during progression of the synchronous cell cycle. Here we demonstrated that c- Fos-like and c-Jun-like proteins participated in G2/M transition by the regulation of the level of Cyclin B 1 protein in P. polycephalum. The study of antibody neutralization revealed that interruption of the functions of c-Fos-like and c-Jun-like proteins resulted in G2/M transition arrest, implicating their functional roles in cell cycle control. When G2/M transition was blocked by histone deacetylase inhibitor trichostatin A, changes in c-Fos- and c-Jun-like protein levels, and hyperacetylation of c-Jun-like protein, were observed. The data suggest that in P. polycephalum, c-Fos- and c-Jun-like proteins may be the key factors in the regulation of histone acetylation-related G2/M transition, involving the coordinated expression and hyperacetylation of these proteins.The homologs of transcription factors c-Fos and c-Jun have been detected in slime mold Physarum polycephalum during progression of the synchronous cell cycle. Here we demonstrated that c- Fos-like and c-Jun-like proteins participated in G2/M transition by the regulation of the level of Cyclin B 1 protein in P. polycephalum. The study of antibody neutralization revealed that interruption of the functions of c-Fos-like and c-Jun-like proteins resulted in G2/M transition arrest, implicating their functional roles in cell cycle control. When G2/M transition was blocked by histone deacetylase inhibitor trichostatin A, changes in c-Fos- and c-Jun-like protein levels, and hyperacetylation of c-Jun-like protein, were observed. The data suggest that in P. polycephalum, c-Fos- and c-Jun-like proteins may be the key factors in the regulation of histone acetylation-related G2/M transition, involving the coordinated expression and hyperacetylation of these proteins.

关 键 词：c-Fos-like protein c-Jun-like protein trichostatin A G2IM transition Physarum polycephalum 

分 类 号：Q51[生物学—生物化学]