人脑源神经营养因子基因cDNA的克隆  

MOLECULAR CLONING OF HUMAN BRAIN-DERIVED NEUROTROPHIC FACTOR

作　　者：程焱[1] 徐国威[1] 张娟[1] 江德华[1] 顾健人[1] 

机构地区：[1]天津医科大学总医院神经内科,天津市神经病学研究所神经化学室,上海市肿瘤研究所癌基因与相关基因国家重点实验室

出　　处：《中华医学遗传学杂志》1996年第2期85-88,共4页Chinese Journal of Medical Genetics

基　　金：上海市科委科技课题项目基金;国家科委攀登计划资助

摘　　要：为研究人脑神经营养因子（ＢＤＮＦ）的生物特性，必须先着手克隆人ＢＤＮＦ基因。作者以人胎脑ｃＤＮＡ为模板，采用ＰＣＲ法得到ＢＤＮＦ基因ｃＤＮＡ探针，标记该探针进一步筛选人胎盘ｃＤＮＡ文库，获得１个１３３５ｂｐ的ｃＤＮＡ克隆ＨＵＭＢＤＮＦＤ。序列测定和分析表明，该克隆含有全长ＢＤＮＦ基因ｃＤＮＡ，基编码区及３′端非编码区与已发表的３个人ＢＤＮＦ基因序列完全同源，而其５＇端非编码区的序列与后三者均有差异，推测可能与组织特异性有关。Neurotrophins are a kind of factor in regulating the growth and survival of selected peripheral and central nervous system.Brain-derived neurotrophic factor(BDNF) is a member of the family.We have obtained a BDNF cDNA clone by screening human placenta cDNA library with a probe which was prepared by PCR with the template of human fetus brain cDNA.The clone human brain-derived neurotrophic factor(HUMBDNFD) was sequenced. It contained 1 33 5 base pairs, After analyzed with GenBank(1993.10.NIH.USA).the HUMBDNFD contained complete coding and 3'untranslated region with homology(100%)to the described three BDNF cDNA sequences(HUMBDNF,HUMBDNFB,HUMBDNFC),but the 5'untranslated region differed from them.

关 键 词：PCR 脑源 神经营养因子 分子克隆 序列测定 

分 类 号：Q343[生物学—遗传学]