大通牦牛生长激素基因和Κ-酪蛋白基因的PCR—RFLP研究  

Study on PCR-RFLP of Growth Hormone Gene and (?)-casein Gene in Datong Yak

作　　者：白文林[1] 郑玉才[2] 尹荣焕[1] 罗光彬[1] 窦全林[3] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]西南民族大学生命科学与技术学院,成都610041 [3]青海大学农牧学院,西宁810003

出　　处：《西北农业学报》2005年第4期1-4,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(39870607)。

摘　　要：利用PCR-RFLP技术检测了大通牦牛生长激素(growth hormone,GH)基因和Κ-酪蛋白(Κ- casein,Κ-CN)基因部分序列的遗传多态性,结果表明:大通牦牛群体中被检测的两个位点均存在遗传多态 性。GH基因PCR—RFLP位点等位基因A和B的基因频率分别为0．1755和0．8255;(?)-CN基因PCR-RFLP 位点等位基因A和B的基因频率分别为0．1117和0．8 883。GH基因和(?)-CN基因PCR-RFLP位点的基因 型分布均极显著偏离Hardy—Weinberg平衡定理(P<0．01)。大通牦牛群体内平均基因一致度和基因多样度分 别为0．7561和0．2439。By the technology of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） , genetic polymorphism of part sequence of growth hormone gene and K-casein Gene in Datong yak was tested. The result showed： the both sites tested were polymorphic in Datong yak. The allele frequencies of A and B were 0. 1755 and 0. 8255 of growth hormone gene PCR-RFLP respectively; and the allele frequencies of A and B were 0. 1117 and 0. 8883 of K-casein gene PCR-RFLP respectively. The genotype distribution of both sites above mentioned in Datong yak didn＇t fit significantly Hardy-Weinberg Law （P 〈 0. 01）. The average gene homozygosity and diversity were 0. 7561 and 0. 2439 respectively in Datong yak.

关 键 词：大通牦牛 GH基因 K-酪蛋白基因 PCR—RFLP 多态性 

分 类 号：S823.85[农业科学—畜牧学]