百合无症病毒CP基因在大肠杆菌中的表达  

The Expression of Lily Symptomless Carlavirus CP Gene in Escherichia Coli

作　　者：李燕[1] 李发慧[1] 陈毓荃[1] 

机构地区：[1]西北农林科技大学生命科学院,陕西杨凌712100

出　　处：《西北农业学报》2005年第4期171-173,共3页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：应用RT-PCR 方法从感病百合总RNA中扩增出百合无症病毒的外壳蛋白基因片段,连接到原核载 体pUC19,转化大肠杆菌DH5α并用IPTG诱导表达。SDS—PAGE及Western blot分析表明,CP基因在大肠肝 菌中获得了特异性表达,融合蛋白分子量约为35kD。The coat protein gene of Lily symptomless carlavirus was amplified by RT-PCR from the extracted RNA of infected lily, and ligated to the expression vector pUC19. The plasmid was transformed into E. coli DH5α and then induced by IPTG. It was shown that the gene was highly and especially expressed by SDS-PAGE and Western blot analysis. The molecular weight of the recombinant protein was about 35kD.

关 键 词：百合无症病毒 外壳蛋白 原核表达 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]