卒中后抑郁大鼠海马及皮层CREBmRNA表达及中药的干预作用  

Expression of CREBmRNA in hippocampus and prefrontal cortex in rats with post-stroke depression and interference effect of Chinese medicine

作　　者：徐向青[1] 唐启盛[2] 侯秀娟[2] 罗斌[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《北京中医药大学学报》2005年第6期35-38,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30171163)

摘　　要：目的观察卒中后抑郁模型大鼠脑内核转录因子CREBmRNA的表达及中药颐脑解郁方的干预作用。方法在卒中后抑郁大鼠模型上,采用逆转录PCR(RT-PCR)方法,对CREBmRNA在卒中后抑郁大鼠脑内前额叶皮质、海马的表达及中药颐脑解郁方的干预作用进行观察。结果卒中后抑郁模型大鼠脑内前额叶皮质、海马CREBmRNA的表达明显低于正常组,中药颐脑解郁方能明显上调CREBmRNA的表达。结论卒中后抑郁模型大鼠脑内CREBmRNA的表达降低,中药颐脑解郁方能使之明显上调,提示卒中后抑郁症CREB表达下降,颐脑解郁方的抗抑郁作用可能与上调CREBmRNA的表达有关。Objective To observe the expression of nuclear transcription factor - cyclic response element - binding protain （CREB） mRNA and the interference effect of Yinaojieyu Formula on the rat model of post - stroke depression. Method Reverse transcription polymerase chain reaction （RT -PCR） was used in the rat model of post - stroke depression. The expression of CREBmRNA in prefrontal cortex and hippocampus, and the interference of Yinaojieyu Formula were observed. Result The expression of CREBmRNA in hippocampus and prefrontal cortex in the rat model of post - stroke depression in the treatment group decreased obviously compared to that of the normal group. Yinaojieyu Formula improved obviously the expression of CREBmRNA. Conclusion The expression of CREB mRNA in the brain of rat model of post - stroke depression decreased, while Yinaojieyu Formula can increases this expression. The result showed that the expression of CREB decreased in post - stroke depression and the anti - depression effect of Yinaojieyu Formula may be related to its function of improving CREBmRNA expression.

关 键 词：卒中后抑郁 核转录因子 颐脑解郁方 

分 类 号：R285.5[医药卫生—中药学]