山西猪种及其杂种群体H-FABP基因的PCR-RFLP研究  

Research on the Polymorphism of Heart Fatty Acid-binding Protein Gene in Shanxi Pig Breeds and Their Crossbred Populations Using PCR-RFLP

作　　者：杨文平[1] 曹果清[1] 薛尚军 刘建华[1] 李步高[1] 周忠孝[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]大同种猪场,大同037000

出　　处：《遗传》2005年第6期882-886,共5页Hereditas（Beijing)

基　　金：山西省科技厅攻关项目资助(编号:991016)~~

摘　　要：利用PCR-RFLP技术对马身猪、山西白猪及其杂种群体共286头猪的心脏脂肪酸结合蛋白(H-FABP)基因5′-上游区(HinfⅠ-RFLP)和第二内含子内(HinfⅠ*-RFLP和HaeⅢ-RFLP)的遗传变异进行了研究。结果表明:(1)在HaeⅢ-RFLP位点上,马身猪均为DD纯合子,而其他猪群在此位点上均存在变异,马身猪的杂种群体在该位点上只有两种基因型(DD、Dd);(2)在5′-上游区的HinfⅠ-RFLP位点上,杜洛克猪×山西白猪的杂种群体只有HH基因型,而其他群体都表现出多态性,马身猪等位基因h的频率为0.9727;(3)在第二内含子内的HinfⅠ*-RFLP位点上,马身猪表现出两种基因型(BB、Bb),等位基因B的频率为0.9667;(4)在HinfⅠ*-RFLP和HaeⅢ-RFLP位点上,所有猪群均处于Hardy-Weinberg平衡状态。The genetic variation in 5′- upstream （Hinf I -RFLP）and the second intron （Hinf I ＊ -RFLP, Hoe Ⅲ-RFLP） of heart fatty acid-binding protein（H-FABP）gene were detected with POR-RFLP in 286 pigs including Mashen, Shanxi white pig and their crossbred populations. The results showed as follows：（ 1 ） Mashen presented only DD genotype while other populations varied, and Mashen crossbred populations had only 2 genotypes（ DD, Dd）at the Hoe Ⅲ-RFLP site;（2）At the Hinf I -RFLP site of the 5′-upstream region, the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. （3）At the Hinf I ＊ -RFLP site of the second intron,only Mashen presented 2 genotypes（ BB, Bb）,and frequency of B allele was 0.9667. （4）At the Hoe Ⅲ-RFLP and Hinf I ＊ -RFLP sites,all populations were in Hardy-weinberg equilibrium.

关 键 词：猪 H-FABP基因 PCR-RFLP 多态性 

分 类 号：S828[农业科学—畜牧学]