催化信标检测端粒DNA序列的初步研究  

Primary Study on Spectrophotometric Detection of Telomeric DNA with Catalytic Beacons

作　　者：曾金红[1] 刘琼[1] 黄开勋[1] 徐辉碧[1] 

机构地区：[1]华中科技大学化学系,湖北武汉430074

出　　处：《南开大学学报（自然科学版）》2005年第4期12-16,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：对催化信标检测端粒DNA的方法进行了初步研究.通过设计具有分子信标和核酶特点的DNA序列,使其在与端粒DNA杂交后,结合氯化血红素成为具有类似过氧化物酶活性的催化信标,催化过氧化氢氧化邻苯二胺,用分光光度法定量检测氧化产物2,3-二氨基吩嗪,其吸光度随端粒DNA浓度的增加而增大.This paper describes a new method for the detection of telomeric DNA based on catalytic beacons that catalyzed the oxidization of O-phenylenediamine by hydrogen peroxide. The catalytic beacon was constructed by the hybridization of target DNA with the molecular beacon, leaving a specific oligonucleotide at the 5r end to form a G-quadruplex in combination with hemin. This catalytic beacon possessed peroxidase-like activity and accelerated the oxidization reaction. The absorbance of 2, 3-diaminophenazine, the oxidized product of Ophenylenediamine,increased with augmenting concentration of target telomeric DNA, i.e. , the enhancing activity of catalytic beacon.

关 键 词：催化信标 过氧化物酶活性 端粒DNA 邻苯二胺 2 3-二氨基吩嗪 

分 类 号：O614.8[理学—无机化学] Q523.4[理学—化学]