NaCS-PDMDAAC微胶囊固定化假单胞菌TS-1138从ATC合成L-半胱氨酸  

Immobilization of Pseudomonas SP.TS1138 in NaCS-PDMDAAC Microcapsule Producing L-Cysteine from ATC

作　　者：金永杰[1] 杨文博[1] 刘忠[1] 白钢[1] 李洋[1] 余养盛[1] 孙丹[1] 

机构地区：[1]南开大学生命科学院微生物系,天津300071

出　　处：《南开大学学报（自然科学版）》2005年第4期119-123,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金资助项目(30470053);天津市重点基金资助项目(05YFJZJC00900)

摘　　要：采用非均相反应制备NaCS(硫酸纤维素钠),考察了反应液中正丙醇-硫酸的最佳比例为25∶35,NaCS和PDMDAAC的最适滴制浓度分别为4%和3%;利用NaCS-PDMDAAC微胶囊对假单胞菌TS-1138进行固定化并进行连续培养,结果表明,菌体能在胶囊内很好的生长和繁殖,连续培养132h后菌浓可达到4.5×1011个/mL胶囊,是在相同条件下游离培养的6倍;固定化菌体以ATC为底物生成L-半胱氨酸的催化活性是游离培养菌体的2.5倍,并且固定化菌体的重复使用能力明显优于游离菌体.重新考察固定化菌体的最适催化反应时间为3h,在一定程度上弥补了微胶囊传质性相对较差的不足.Based on the heterogenetic reaction, a process of preparation of NaCS（Sodium Cellulose Sulfate） was proposed. The optimum proportion of optal-sulfuric acid which was studied in all the effects on the synthesis of NaCS was 25 - 35. The suitable concentrations of NaCS and PDMDAAC in the course of dropping were 4% and 3% respectively. Pseudornonas sp. TS-1138 was immobilized in the NaCS-PDMDAAC microcapsule and cultured continuously. The experiment showed that the cells could grow well. In 136 hours, the density of cells could be 4.5 × 10^11 个/mL capsule which was 6 folds than that in free cell culture at the same condition, and the enzyme activity from ATC to L-cysteine was 2. 5 folds. The microencapsulated cells is clearly superior to the free cells when they were applied repeatedly. The optimum catalytic reaction time by the microencapsulated cells is 3 hours, which partly make up the shortcoming of mass transfer by microcapsule.

关 键 词：固定化 NaCS-PDMDAAC微胶囊 假单胞菌 L-半胱氨酸 

分 类 号：Q814.2[生物学—生物工程]