大麦籽粒β-淀粉酶活性的棱型效应和灌浆期间的动态研究  

Differences among barley row types in β-amylase activity and its dynamics during grain development.

作　　者：陈锦新[1] 戴飞[1] 韦康[1] 张国平[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2005年第6期709-713,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：高等学校博士学科点专项科研基金(20020335028)资助;国家自然科学基金(30270779)资助;国家自然科学基金(30471022)资助;科技部863"项目(2001AA241241)资助

摘　　要：测定了不同棱型的188个大麦品种的籽粒β-淀粉酶活性和蛋白质含量,分析比较不同棱型间β-淀粉酶活性的差异,并观察了籽粒灌浆期间β-淀粉酶活性的动态.结果表明,同一棱型的品种间籽粒β-淀粉酶活性均存在较大变异,六棱大麦变异系数最大.多数大麦品种的β-淀粉酶活性变动在500～1500U/g之间,二棱大麦的平均值明显高于四棱和六棱大麦.β-淀粉酶活性与籽粒蛋白质含量之间,二棱大麦品种存在极显著的正相关,而四棱和六棱大麦品种的相关不显著.筛选到8个β-淀粉酶活性高于1500 U/g且籽粒蛋白质含量低于11.5%的二棱型大麦品种.β-淀粉酶活性在籽粒灌浆前期和后期增加幅度明显高于中期,最高值出现在成熟期,与单位籽粒蛋白质积累量呈显著正相关.Grain β-amylase activity and protein content of 188 barley genotypes with different row type were determined and the dynamics of β-amylase activity during grain development was measured. The results showed that there was a wide Variation in grain β-amylase activity among varieties with the same row type, six-rowd type had the highest coefficient of variation among three types of genotypes. β- amylase activity in most barley varieties ranged from 500 U/g to 1500 U/g, and on average of all varieties with the same row type, two-rowed barleys had the highest β-amylase activity. Highly significant positive correlation between β-amylase activity and protein content was found for two-rowed barleys, but not four- and six-rowed barleys. Eight reasonable varieties with β-amylase activity above 1500 U/g and protein content below 11.5% were identified from two-rowed barleys. β-amylase activity showed more rapid increase at early and late grain-filling stage than at medium stage, and the highest activity occurred just at maturity stage. In addition, β-amylase activity was significantly and positively correlated with protein content in the grains during grain development.

关 键 词：大麦 β-淀粉酶活性 棱型 动态 

分 类 号：S512.3[农业科学—作物学]