肌红蛋白基因多态与有氧训练效果的关联性分析  

Association between Myoglobin Gene Polymorphism and Physical Performance after Endurance Training

作　　者：吴剑[1] 胡扬[1] 刘刚[1] 周多奇[1] 席翼 文立[2] 

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]天津体育学院运动人体科学系

出　　处：《中国运动医学杂志》2005年第6期650-654,共5页Chinese Journal of Sports Medicine

基　　金：国家科技部课题(项目编号:2003BA904B04)资助

摘　　要：目的:探讨肌红蛋白第2外显子的单核苷酸多态(A79G)与有氧耐力训练效果的关联性。方法:对中国北方新征入伍的104名汉族士兵进行每次5000米、每周3次、训练强度为95～105%通气无氧阈(VT)、为期20周的耐力训练。递增负荷运动实验测定受试对象训练前后最大摄氧量(VO2max)和通气无氧阈(VT),PCR-RFLP法测定基因多态性,分析基因多态性与训练效果之间的关联性。结果:3种基因型的分布频率分别为AA(0.52)、AG(0.45)、GG(0.03),分布频率符合Hardy-weinberg遗传平衡定律;训练后3种基因型VO2max的组间比较均无显著性差异(P>0.05);训练后GG组和AG组VT显著提高(P<0.05),AA组达到VT时仅HR显著升高,其余指标均无显著变化(P>0.05)。在VO2max和VT的变化率(Δ%)方面,GG组受试者提高最多,AG组居中,AA组提高最少。提示肌红蛋白基因第2外显子的单核苷酸多态(A79G)与有氧耐力训练效果有一定关联,79G等位基因携带者对有氧耐力训练可能更敏感。Objective To explore the association between the allele frequency of the single nucleotide polymorphism （A79G） in the second exon of the myoglobin gene and physical performance. Methods 104 male soldiers, underwent a distance running program, which consisted of 5000m running with the intensity of 95- 105% ventilatory threshold （VT）, 3 times/week for 20 wk. VO2max and VT were measured on a treadmill before and after the running program, and myoglobin gene polymorphism was detected by PCRRFLP. Results The genotype frequencies were as follows- AA = 0.52, AG = 0.45, GG = 0.03 and they were coincident with Hardy - Weinberg equilibrium. No significant changes in three genotypes of the second exon of myoglobin gene were found （P 〉 0.05）. VT increased significantly in subjects with AG and GG genotypes. In AA, HR at VT was the only parameter that increased significantly after the running program. The subjects with GG genotype, moreover, increased most in AVO2max% and △VT%, and AA increased least. The present study demonstrated that the allele of G in the second exon of myoglobin gene might contribute to individual differences in endurance training.

关 键 词：肌红蛋白基因多态 有氧耐力训练 VO2MAX VT 

分 类 号：R87[医药卫生—运动医学]