运动疲劳对大鼠新纹状体神经元电活动的影响  

Changes in Spontaneous Activity of Neostriatal Neurons in Rat after Exercise-induced Fatigue

作　　者：乔德才[1] 侯莉娟[1] 何德富[2] 翁恩琪[2] 

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]华东师范大学环境科学系生理组

出　　处：《中国运动医学杂志》2005年第6期676-680,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(项目编号:30470832);山西省自然科学基金(项目编号:20051116)资助

摘　　要：目的:观察运动疲劳大鼠新纹状体神经元自发放电情况,探讨运动疲劳产生的中枢机制。方法:采用胞外玻璃微电极技术,对运动疲劳前后大鼠新纹状体神经元自发放电频率、神经元动作电位时程及动作电位发放形式进行记录,并对放电神经元的分布规律进行分析。结果:(1)在记录到的运动疲劳组大鼠新纹状体神经元中,19%自发放电频率>10Hz,而对照组仅有6%,两组相比存在显著差异(P<0.05);(2)运动疲劳组大鼠新纹状体神经元除观察到规则单脉冲放电、不规则单脉冲放电、单脉冲与爆发式并存的放电形式外,还观察到规则爆发式放电,其串间隔集中在140～210ms;(3)运动疲劳组高频自发放电的神经元主要集中在新纹状体的外侧深部区域。结论:运动疲劳后新纹状体神经元自发放电频率发生改变,高频放电神经元数量明显增加。结果提示运动疲劳后神经元放电形式发生改变,可能与神经元胞内钙离子浓度升高相关。Extracellular records in vivo were made from striatum neurons in male rats after overload exercise. The results showed that （1）Only 6% of spontaneous firing frequency in neostriatum neurons in control group was above 10Hz, whereas 19% of spontaneous firing frequency in neostriatum neurons in exercise- induced fatigue group was above 10Hz. The difference between firing frequencies in two groups was significant. （2） In exercise - induced fatigue group, we recorded not only regular firing, irregular and regular - irregular firing but also regular bursting firing and the time between the bursts was 140 -210ms. （3） In exercise group, the high frequency neurons were mainly focused in the dorsal part of the neostriatum. From the results, we concluded that the neostriatal neurons discharge frequency was changed due to exercise- induced fatigue, and the high frequency neuron＇s percentage increased significantly. The changes in the firing pattern directly relat- ed to the concentration of intracellular Ca^2＋ .

关 键 词：运动疲劳 新纹状体 胞外记录 玻璃微电极技术 电生理学方法 

分 类 号：R87[医药卫生—运动医学]