生理状态下外周血白细胞γ-干扰素基因表达的荧光定量PCR检测  

Quantitative Real-time Detection of Spontaneous Interferon-γ Gene Expression in Peripheral Blood Mononuclear Cell (PBMC) by Polymerase Chain Reaction

作　　者：王茹[1] 陈佩杰[1] 董强刚[1] 

机构地区：[1]上海体育学院运动科学系,上海200438

出　　处：《中国运动医学杂志》2005年第6期693-695,698,共4页Chinese Journal of Sports Medicine

基　　金：国家科技攻关计划(2002BA904B04-6);上海市教委第四期重点学科--运动人体科学学科建设项目;上海市第二期重点学科(T0901);国家教育部高等学校优秀青年教师教学科研奖励计划;上海市体育局科研攻关项目共同资助

摘　　要：目的:建立γ-干扰素(IFN-γ)基因表达的绝对定量实时PCR检测技术。方法:应用实时定量PCR方法检测25名健康运动员生理状态下外周血白细胞IFN-γmRNA的表达。主要结果和结论:应用实时定量PCR方法能够对健康受试者外周血白细胞自发性IFN-γmRNA表达进行定量分析,相关系数r>0.99。Objective To develop a method for quantitative real- time detection of interferon- γ （IFN - γ） using polymerase chain reaction （PCR）. Methods Gene expression of IFN - γ in peripheral blood mononuclear cell （PBMC） from 25 healthy individuals was detected by PCR. An endogenous “housekeeping” gene GAPDH was used to normalize the results. Results and Conclusion The spontaneous IFN- γ mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r 〉 0.99.

关 键 词：Γ-干扰素 信使核糖核酸 实时定量PCR 生理状态 

分 类 号：R87[医药卫生—运动医学]