芽孢杆菌HBS4还原亚硒酸钠为单质硒的实验研究  

Sodium selenite reduction to elemental selenium by Bacillus HBS4

作　　者：李瑞萍[1] 鲁安怀[1] 郝瑞霞[1] 马黠静[1] 吕明[1] 鲁志强[1] 

机构地区：[1]北京大学地球与空间科学学院,北京100871

出　　处：《岩石矿物学杂志》2005年第6期598-602,共5页Acta Petrologica et Mineralogica

基　　金：国家重大基础研究前期研究专项资助项目(2001CCA02400)

摘　　要：利用从油藏中分离的芽孢杆菌HBS4在兼性厌氧情况下作用于剧毒物质亚硒酸钠,36h后1～11mM的样品中均出现了大量红色颗粒沉淀物。生物显微镜观察发现,在很多细菌内部出现一颗到多颗红色的圆形颗粒,排列方式有单链、双链、弧线和无规则排列,并且在细胞周围释放有游离态的红色颗粒。XPS测试红色沉淀物发现四价硒全部还原为零价硒。实验还发现添加葡萄糖对单质硒的产量有很大的促进作用。The experiments in this paper employed the strains of Bacillus HRS4 isolated from oil reservoir. By growing in the facultatire aerobic condition the cells acted on selenite. After 36 hours, all the samples with sodium selenite radical from 1mM up to 11mM produced many red deposits. Under biomicroscope the authors found one or more red round intracellular deposits in the cell with free floating deposits outside the cell. Intracellular organization of deposits includes one or two regular chains, curve or irregular in form. The XPS patterns of the deposits show that the sodium selenite has been exclusively reduced to elemental selenium. Furthermore, glucove in the culture media can impact greatly the quantity of the deposits of the reduced elemental selenium.

关 键 词：微生物 芽孢杆菌HBS4 亚硒酸钠 单质硒 生物缓毒 

分 类 号：Q939.1[生物学—微生物学] P579[天文地球—矿物学]