离子液体反胶团中青霉素酰化酶的水解反应特性  

Penicillin acylase hydrolysis of 6-APA in reversed micelle with ionic liquids

作　　者：夏寒松[1,2] 余江[1] 刘会洲[1] 

机构地区：[1]中国科学院过程工程研究所分离科学与工程实验室,生化工程国家重点实验室 [2]中国科学院研究生院,北京100039

出　　处：《化工学报》2005年第10期1932-1935,共4页CIESC Journal

基　　金：国家自然科学基金项目(20236050);创新群体(20221603).~~

摘　　要：Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with [C14mim]Cl ionic liquids. The experi ment indicated that this catalytic reaction showed better performance in enzymat ic solubility, activity and stability than the reaction in reversed micelle with cetyl trimethyl ammonium bromide （CTAB）. For example, the maximum solubility of penicillin acylase in ionic liquids system was twice more than that in CTAB, an d the maximal enzymatic activity in ionic liquids was 1.3 times than that in CT AB.The outstanding properties of ionic liquids, including low charge density and irregular sharpness of head ring,might be attributable to its distinct head gro up. Ionic liquids made it possible to optimize the enzymatic catalysis in rever sed micelle through altering its interfacial structure effectively.Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with [C14 mim]Cl ionic liquids. The experiment indicated that this catalytic reaction showed better performance in enzymatic solubility, activity and stability than the reaction in reversed micelle with cetyl trimethyl ammonium bromide （CTAB）. For example, the maximum solubility of penicillin acylase in ionic liquids system was twice more than that in CTAB, and the maximal enzymatic activity in ionic liquids was 1.3 times than that in CTAB. The outstanding properties of ionic liquids, including low charge density and irregular sharpness of head ring, might be attributable to its distinct head group. Ionic liquids made it possible to optimize the enzymatic catalysis in reversed micelle through altering its interracial structure effectively.

关 键 词：离子液体 反胶团 青霉素酰化酶 青霉素水解反应 6-氨基青霉烷酸 

分 类 号：TQ464.8[化学工程—制药化工] Q556.4[生物学—生物化学]