穿心莲药材的色谱指纹图谱  

The Fingerprints of Andrographis paniculata by HPLC/UV/MS

作　　者：张尊建[1] 董海娟[1] 余静[2] 

机构地区：[1]中国药科大学分析测试中心,南京210009 [2]南京医科大学公共卫生学院,南京210029

出　　处：《中国天然药物》2005年第6期373-376,共4页

基　　金："十五"国家重大科技专项"创新药物和中药现代化"资助项目(2001BA701A56)~~

摘　　要：目的:建立穿心莲药材的HPLC/UV色谱指纹图谱,并对不同产地的药材进行比较。方法:应用Shimadzu VP-ODS柱,0.1%甲酸乙腈与0.2%甲酸进行梯度洗脱,流速为1.0 ml/min,检测波长254 nm,柱温为室温。结果:得到分离度、重现性均较好的穿心莲药材HPLC/UV指纹图谱,标示了9个共有峰。应用HPLC/MS技术对其7个主要色谱峰进行了初步归属,并对不同产地药材的色谱指纹图谱进行了相似度评价。结论:穿心莲药材指纹图谱的建立,为其质量控制提供了依据。AIM： To establish the chromatographic fingerprints of Andrographis paniculata （Burro. f. ） Nees. by HPLC/UV and compare the fingerprints of Andrographis paniculata collected from different areas. METHOD： Separation was carried out on a Shimadzu VP-ODS column. Gradient elute was performed by the mobile phase consisting of 0.1% formic acidacetonitrile and 0.2% formic acid- water with the flow rate of 1.0 mL/min. The column temperature was set at 25 ℃ and detecting wavelength at 254 nm. RESULT： Perfect fingerprints were obtained which can be used for the evaluation of Andrographis paniculata. Nine common peaks were confirmed in fingerprints. Seven compounds were elucidated by HPLC/MS. CONCLUSION： The method can be applied for the quality control of Andrographis paniculata.

关 键 词：穿心莲 指纹图谱 HPLC/UV/MS 化学成分 

分 类 号：TQ460.72[医药卫生—药物分析学]