HFRS疫苗佐剂吸附前后加入保护剂对其免疫原性的影响  

Comparison the effects of stabilizer to immunogenicity of HFRS bivalent purified vaccine before and after aluminum adjuvant absorption

作　　者：岳立广[1] 马军[1] 韩亮[1] 惠连[1] 赵丽红[1] 张龙梅[1] 李彤[1] 王贵斌[1] 柴义[1] 宋宗明[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《微生物学免疫学进展》2005年第4期19-21,共3页Progress In Microbiology and Immunology

摘　　要：为提高肾综合征出血热(HFRS)双价纯化疫苗抗原的有效利用率,在保证疫苗免疫效果的前提下,合理地降低疫苗的成本,确定最佳疫苗配制方法和最合适的疫苗浓缩倍数。将抗原含量不同的出血热纯化疫苗原液及疫苗配制的两种主要成分———人血白蛋白和A l(OH)3佐剂按不同的配制顺序配制成4批纯化疫苗,分别抽样,测其上清液中抗原量并做效力试验。结果表明人血白蛋白和A l(OH)3佐剂加入的顺序不同,配制的4批纯化疫苗上清液抗原量也不同,效力试验结果显示其中和抗体阳转率(PRNT)也有很大差别。因此在纯化疫苗的配制过程中,人血白蛋白和A l(OH)3的加入顺序影响到佐剂A l(OH)3对疫苗抗原的吸附,也影响到疫苗的免疫效果;先加A l(OH)3再加人血白蛋白的疫苗免疫效果要好于先加人血白蛋白再加A l(OH)3的疫苗。To increase the antigen＇s utilization and reduce costs of production, but not to lower the quality, and finally determine the optimal formulation for haemorrhagic fever with renal syndrome bivalent purified vaccine （ HFRS vaccine ）. Four lots of HFRS vaccine were prepared in a special order with the different amount of antigen, of human serum albumin （HSA） and of Al（OH）3 adjuvant. The sampling were carried out to examine the antigen content in the supernatant and to do the potency test. There were some differences in antigen content in the supernatant, and a distinct positive conversion rate of neutralizing antibody （ PRNT ） existed in the four lots, with the different preparing order of HSA and Al（OH）3. It is superior to obtain a better immunogenicity for HFRS vaccine by first adding Al（OH）3 and then HSA.

关 键 词：肾综合征出血热双价纯化疫苗 佐剂 抗原 免疫效果 

分 类 号：R392.1[医药卫生—免疫学]