新城疫HA-HI试验中可用豚鼠醛化红细胞代替鸡新鲜红细胞  

Hydroformylated Guinea pig red blood cell can substitute fresh chicken red blood cell in the HA-HI test for Newcastle Disease

作　　者：黄兰香[1] 李建新 尹振海[1] 潘国庆[1] 

机构地区：[1]西南大学动物科技学院,重庆400716 [2]成都市兽医卫生检疫站,四川成都610041

出　　处：《家禽科学》2005年第12期18-19,共2页Poultry Science

摘　　要：在鸡新城疫血凝(HA)和血凝抑制(HI)试验中,将豚鼠红细胞用戊二醛醛化处理后配制成2%细胞悬液,与2%新鲜豚鼠红细胞、1%新鲜鸡红细胞获得的检测结果基本一致。经醛化的豚鼠红细胞在4℃或室温条件下也能长时间保存而不溶血。结果表明,在新城疫HA-HI试验中可以用豚鼠醛化红细胞代替鸡新鲜红细胞。In the hemagglutination（HA） and hemagglutination inhibition（HI） test for chicken Newcastle disease （ND） virus antigen and specific antibody, the identical results could be obtained using 2% suspensions of Guinea pig red blood cell （RBC） treated with glutaraldehyde,2% fresh Guinea pig RBC and 1% fresh chicken RBC.The hydroformylated Guinea pig RBC can be preserved at 4℃ and room temperature for longer time than fresh RBC and no erythrolysis occurred. The result indicated that 2% hydroformylated RBC of guinea pig could substitute the chicken RBC in HA-HI test for ND.

关 键 词：新城疫 豚鼠 醛化红细胞 血凝和血凝抑制试验 

分 类 号：S854.43[农业科学—临床兽医学]