不同菜用大豆品种的胚胎发生与再生  

EMBRYOGENESIS AND REGENERATION FROM DIFFERENT OF VEGETABLE SOYBEAN

作　　者：王萍[1] 王罡[2] 季静[2] 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]天津大学农业与生物工程学院,天津300072

出　　处：《大豆科学》2005年第4期314-316,共3页Soybean Science

基　　金：国家植物转基因中试及产业化基地专项基金(项目编号J99-B-001)

摘　　要：以8个菜用大豆品种的未成熟子叶为外植体,在MS(附加高浓度2,4-D)培养基诱导愈伤组织形成、体细胞胚胎发生与再生植株.结果表明:八个菜用大豆品种的未成熟子叶均可诱导产生愈伤组织与体细胞胚胎发生,出愈率为14.71%～100%.体细胞胚胎发生率变化在0～36.76%之间,绿子叶菜用大豆没有形成体细胞胚.体细胞胚可正常萌发产生小植株,移栽成活结实.菜用大豆的出愈率和体细胞胚胎发生率与基因型有关.Callus formation, somatic embryogenesis and plant regeneration were induced by MS medium including higher concentrations of 2,4-D with immature cotyledon of eight genotypes in vegetable soybean. The results showed that callus formation and somatic embryogenesis of eight genotypes of vegetable soybean with immature cotyledon were induced successfully. Frequencies of callus formation varied from 14.7% to 100% and frequencies of somatic embryogenesis changed 0-36.76%. Somatic embryo has not been induced from variety Luziyecaidou with green cotyledon. Somatic embryos could germinate, regenerate plantlets and seed. The frequencies of callus and somatic embryogenesis were associated with genotypes in vegetable soybean.

关 键 词：菜用大豆 未成熟子叶 胚胎发生 再生植株 

分 类 号：S643.7[农业科学—蔬菜学]