山茱萸环烯醚萜总苷对糖尿病大鼠肾形态学及其Na^+,K^+-ATP酶活性的影响  

Influence of Iridosides of Cornus Officinalis on the Renal Morphology and It's Na^+,K^+-ATPase Activity of Diabetic Rats

作　　者：李丽华[1] 许惠琴[1] 时艳[1] 

机构地区：[1]南京中医药大学药理教研室,江苏南京210029

出　　处：《云南中医学院学报》2005年第4期43-45,共3页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：江苏省科技厅自然科学基金资助项目(NO:BK2001127)

摘　　要：目的:从形态学及蛋白酶的角度探讨山茱萸环烯醚萜总苷(ICO)防治糖尿病肾病(DN)的作用。方法:腹腔注射链脲佐菌素造成糖尿病大鼠模型,将造模成功的大鼠按血糖值随机分为模型组、氨基胍组和ICO组,同期另设一正常组,各组连续灌胃12周。实验结束后用光镜观察肾形态学变化,试剂盒测定肾组织细胞膜Na+,K+-ATP酶的活性。结果:模型组大鼠肾脏光镜检查肾小球和肾小管病变明显。而且肾组织细胞膜Na+,K+-ATP酶活性低于正常组。而ICO组病变明显减轻,表现出较好的抗肾小球内基质增生、抗肾小管坏死和改善Na+,K+-ATP酶活性等作用。结论:ICO可改善糖尿病大鼠肾组织形态学病变及修复Na+,K+-ATP酶活性,对DN所引起的结构和功能改变都具有一定的防治作用。Aim To study the protective effect of iridosides of comus officinalis （ICO） on diabetic nephropathy （DN） from the renal morphology and protease. Methods： Diabetic rats were induced by ip. Streptozotoin, The model rats were divided randomly into three groups： control, aminoguanidine and ICO. One more normal group was added. Those rats were fed with each drug for twelve weeks. The morphology of kidney were observed under light microscope. The Na ^＋ , K^ ＋ - ATPase activity were tested by Na^ ＋ , K^ ＋ - ATPase test Kit. Result： The model group showed apparent pathological changes in glomerulus and renal tubule. The Na^ ＋ , K ^＋ - ATPase activity in model groups remarkably decreased. While ICO can relieve mesangial matrix hyperplasia, prevent renal tubular from necrosis and recover Na ^＋ , K^ ＋ - ATPase activity. Conclusion： From the ameliorative role of ICO on renal morphology and Na^ ＋ , K^ ＋ - ATPase activity, we conclude that it should be beneficial to the prevention of structural and functional damage of the diabetic nephropathy

关 键 词：山茱萸环烯醚萜总苷 糖尿病肾病 形态学 NA^+ K^+-ATP酶 

分 类 号：R285.6[医药卫生—中药学]