海马神经元中TAT-GluR6-9c-dansyl小肽的荧光观察  

Delivery of GluR6-9c into hippocampal neurons by TAT transduction domain

作　　者：纵艳艳[1] 裴冬生[1] 孙亚峰[1] 张光毅[1] 

机构地区：[1]徐州医学院生物化学与分子生物学研究中心,江苏徐州221002

出　　处：《徐州医学院学报》2005年第6期488-490,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金资助项目(30330190)

摘　　要：目的研究TAT-GluR6-9c-dansyl荧光小肽是否可以进入细胞内部。方法在培养的海马神经元中加入TAT-GluR6-9c-dansyl荧光小肽和对照肽TAT38-48-dansyl。结果用10μmol/L TAT-GluR6-9c-dansyl处理的海马神经元在荧光显微镜下可以观到绿色荧光的出现,而对照肽TAT38-48-dansyl无荧光出现。结论TAT-GluR6-9c-dansyl荧光小肽可以进入细胞。Objective To test whether TAT- GluR6- 9c could be delivered into hippocampal neurons. Methods The target and control peptides were separately incubated with cultured hippocampal neurons and their fluorescence was visualized by fluoroscopy. Results Neurons treated with TAT - GluR6 - 9c - dansyl （ 10 μmol/L） exhibited strong fluorescence in the cytoplasm, indicating the uptake of intracellular peptide, whereas in the cultures treated with TAT38 - 48 - dansyl, lacking the transduction domain, only background signals were observed, indicating absence of peptide uptake. Conclusion TAT - GluR6 - 9c could be delivered into hippocampal neurons.

关 键 词：TAT GLUR6 DANSYL 海马神经元 

分 类 号：Q593.2[生物学—生物化学]