高效液相色谱-电喷雾串联四极杆质谱法研究人尿中毛果芸香碱代谢产物  

Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

作　　者：王玲[1] 王英武 陈刚[1] 袁宏明 

机构地区：[1]吉林大学测试科学实验中心 [2]生命科学学院药物代谢研究中心 [3]化学学院,长春130023

出　　处：《高等学校化学学报》2005年第12期2230-2232,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20471021)资助

摘　　要：Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer.The urine sample was collected from 0 to 8 h after oral administration. The urine sample was prepared by solid phase extraction,and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode. Three metabolites were identified as the oxidative metabolite,pilocarpic acid and the glucuronide of pilocarpine.Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer. The urine sample was collected from 0 to 8 h after oral administration. The urine sample was prepared by solid phase extraction, and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode. Three metabolites were identified as the oxidative metabolite, pilocarpic acid and the glucuronide of pilocarpine.

关 键 词：毛果芸香碱 代谢物 液相色谱-串联质谱法 

分 类 号：O657[理学—分析化学]