N-甲基吡咯类聚酰胺的合成及其与DNA的相互作用  

Synthesis of Polyamides Containing N-Methylpyrrole Rings and Their Interaction with DNA

作　　者：李钦玲[1] 周江[1] 刘丹[1] 袁谷[1] 

机构地区：[1]北京大学化学学院化学生物学系生物有机与分子工程教育部重点实验室

出　　处：《高等学校化学学报》2005年第12期2267-2269,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20272005)资助

摘　　要：Polyamides containing N-methylpyrrole and N-methylimidazole amino acids have attracted considerable attention from biological chemists because they can permeate living cell membrane and have the potential to control specific gene expression.In this article,three polyamides containing N-methylpyrrole rings were synthesized by the chloroform reaction and the DCC/HOBT coupling reaction,and the interaction between the polyamides and DNA was investigated by electrospray ionization mass spectroscopy.The results of the experiments indicate that the designed polyamides can effectively bind the target DNA,and ESI mass spectrometry is an excellent tool for the analysis of the interactions between DNA and the polyamides.Polyamides containing N-methylpyrrole and N-methylimidazole amino acids have attracted considerable attention from biological chemists because they can permeate living cell membrane and have the potential to control specific gene expression. In this article, three polyamides containing N-methylpyrrole rings were synthesized by the chloroform reaction and the DCC/HOBT coupling reaction, and the interaction between the polyamides and DNA was investigated by electrospray ionization mass spectroscopy. The results of the experiments indicate that the designed polyamides can effectively bind the target DNA, and ESI mass spectrometry is an excellent tool for the analysis of the interactions between DNA and the polyamides.

关 键 词：聚酰胺 电喷雾离子化质谱 DNA识别 

分 类 号：O627.7[理学—有机化学]