茶毛虫NPV的P24、Rr1、Lef1基因及其分子进化分析  

Phylogenetic Relationship of Tea Tussork(Euproctis pseudoconspersa) Nucleopolyhedrovirus and Other Baculoviruses Based on P24、Rr1 and Lef1 Genes

作　　者：聂婷婷[1] 肖强[2] 殷坤山[2] 邢丽苹[1] 张传溪[1] 

机构地区：[1]浙江大学应用昆虫学研究所,浙江杭州310029 [2]中国农业科学院茶叶研究所,浙江杭州310008

出　　处：《茶叶科学》2005年第4期242-248,共7页Journal of Tea Science

基　　金：国家自然科学基金项目(30070032);全国优秀博士学位论文作者专项基金(200055);浙江省科技计划(2003C32003)

摘　　要：克隆和测定了茶毛虫核型多角体病毒(EupsNPV)全长5942bp的EcoRI-XhoI基因组DNA片段。序列分析表明该片段包含5个完整的开放阅读框(ORF),分别编码P24、Rr1、38.7K、Lef1和Ep-ld124。通过对上述5个基因与其它已知杆状病毒的同源基因的分析比较,以及对P24、Rr1和Lef1的分子进化树分析,表明EupsNPV是一种与已知杆状病毒都有较大差异的病毒种类,属于NPVII组病毒。结果还表明EupsNPV与茶尺蠖(Ectropisobliqua)核多角体病毒(EcobSNPV)的亲缘关系最为接近。本文报道的序列的EMBL登录号为AJ920288。An EcoRI-XhoI 5942 bp DNA fragment from the Euproctis pseudoconspersa nucleopolyhedrovirus （EupsNPV） genome was cloned and its sequence was determined. Five Open Reading Frames （ORFs） encoding P24, Rrl, 38.7 K, Lefl and Ep-ld124 were identified in the fragment. Comparative and phylogenetic analysis of the 5 genes with their homologues from other baculoviruses revealed that EupsNPV was a nucleopolyhedrovirus distinct from other known baculoviruses. The result also showed that EupsNPV was one of the NPV II members, and was most closely related to Ectropis obliqua SNPV （EcobSNPV）. The sequence data reported in this paper have been assigned the EMBL accession number AJ920288.

关 键 词：茶毛虫 核型多角体病毒 P24 lef1 Rr1 序列分析 分子进化 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]