重组人造血增效因子对猴外周血干细胞的快速动员作用  

The effect of rhHSF on rapid mobilization of peripheral blood hematopoietic stem cells

作　　者：谢玲[1] 刘耀文[1] 齐春梅 熊国林[1] 王欣茹[1] 黄海潇[1] 余祖胤[1] 刘萍[3] 杨洁[3] 郝静[1] 董波[1] 陈松森[2] 罗庆良[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]中国医学科学院基础医学研究所 [3]解放军总医院药材处

出　　处：《解放军医学杂志》2005年第11期990-992,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家"863"计划(编号2001AA215401);全军"十五"指令基金(编号01L019)资助课题

摘　　要：目的观察重组人造血增效因子(rhHSF)对外周血造血干/祖细胞的快速动员作用。方法4只正常成年雄性猕猴连续4天皮下注射重组人粒细胞集落刺激因子(rhGCSF)10μg/(kg·d)后,第5天用rhHSF250μg/kg一次皮下注射。另4只正常成年雄性猕猴仅皮下注射rhHSF500μg/kg一次。观察两组外周血造血干细胞的动员效果。结果一次皮下注射rhHSF后45min、1h和3h,外周血CD34+细胞、CFUGM和中性粒细胞数明显升高并达高峰,其幅度分别为药前值的1.8、3.3和24.9倍。单独应用rhGCSF后4天,对外周血CD34+细胞、CFUGM和中性粒细胞数分别为基值的34.8、4.2和2.7倍;联合应用rhHSF和rhGCSF的动员作用更加明显,CD34+细胞、CFUGM数和中性粒细胞数分别增至基值的41.3、8.3和4.9倍。结论rhHSF能快速动员造血干/祖细胞和中性粒细胞至外周血,并与rhGCSF有明显的协同作用。Objective To evaluate the effects of recombinant human hematopoietic synergistic factor （rhHSF） on rapid mobilization of the peripheral blood stem cells. Methods 8 monkeys were divided into rhG-CSF 10μg/（kg·day） and rhG-CSF 10μg/（kg · day） ＋rhHSF 500μg/（kg · day） group, with 4 monkeys in each group. All of them received rhG-CSF subcutaneously once daily for 4 days, and in the latter group, the animals received rhHSF subcutaneously in single dose on the fifth day. Resdts The highest counts of CD34^＋ cell, CFU-GM and neutrophils in animals receiving rhHSF only were 23, 1.8 and 3.3 times of baseline value at 180, 45 and 60 min, while those in animals receiving rhG-CSF and rhHSF were 41. 3, 8. 3 and 4. 9 times of base line value. Conclusion rhHSF can rapidly mobilize hematopoietic stem cells and neutrophils into peripheral blood, and it is found to synergize with G-CSF to augment stem cell mobilization, suggesting a potent clinical utility of rhHSF in peripheral blood stem cells transplantation.

关 键 词：重组人造血增效因子 重组人粒细胞集落刺激因子 外周血干细胞 

分 类 号：R973[医药卫生—药品]