7条印迹基因在人类种植前胚胎的表达  

The expression of seven imprinted genes in human preimplantation embryos

作　　者：沈文洁[1] 邢福祺[2] 孔令红[2] 陈士岭[2] 李红[2] 

机构地区：[1]解放军总医院304临床部妇产科,北京100037 [2]南方医科大学南方医院生殖中心

出　　处：《解放军医学杂志》2005年第11期1016-1018,共3页Medical Journal of Chinese People's Liberation Army

基　　金：广东省科技计划项目资助(编号2003c34212)

摘　　要：目的完善印迹基因在人类种植前胚胎的表达图谱。方法应用巢式RTPCR技术检测7条印迹基因P57KIP2、LIT1、TSSC3、GRB10、PEG3、ARHI、ZAC1在人类卵子和种植前胚胎中的正常表达。结果卵子和各期种植前胚胎、囊胚中均存在P57KIP2、ZAC1的表达;LIT1自8细胞胚胎开始表达,持续至囊胚期;TSSC3于卵子及各期种植前胚胎中均未表达;4细胞、8细胞胚胎、囊胚及卵子中均可测到GRB10正常表达;除4细胞胚胎未见表达外,卵子和其余各期胚胎均可测得ARHI表达;Peg3表达于4细胞、8细胞胚胎和囊胚。结论除TSSC3外的其余6条基因均表达于种植前胚胎,有助于开展种植前遗传学诊断,为探讨印迹基因与辅助生殖、先天性印迹疾病以及肿瘤的关系提供理论依据。bjective To perfect gene profile expressed in pre-implantation embryos. Methods Using nested RT-PCR to investigate the expression of seven imprinted genes： P57^KIP2, LIT1, TSSC3, GRB10, PEG3, ARHI, and ZAC1 in human oocytes and preimplantation embryos. Results Transcripts of P57^KIP2 and ZAC1 were detected in human oocytes and at all stages of pre-implantation; LIT1 was expressed only in stages of 8-cell and blastocyst; transcripts of TSSC3 could not be detected; GRB10 mRNA could be detected in oocytes and pre-implantation embryos except for E-cell embryo; ARHI was expressed in oocytes and 2,8-cell embryos and blastocyst; Peg3 mRNA existed in 4,8-cell embryos and blastocyst. Conclusion Except for TSSC3, transcripts of the other six imprinted genes are detected in human pre-implantation development, which are helpful for pre-implantation diagnosis of imprinted diseases, and provide the theoretical basis for understanding the correlation among assisted reproductive technology, genetic imprinted diseases and tumor.

关 键 词：印迹基因 种植前胚胎 辅助生殖 基因表达 遗传性印迹病 

分 类 号：R715.5[医药卫生—妇产科学]