鸭病毒性肠炎病毒CH强毒株感染鸭胚成纤维细胞的超微结构观察  

Morphological Observation of Virulent Duck Enteritis Virus Strain CH-Infected Duck Embryo Fibroblasts by Transmission Electron Microscopy

作　　者：郭宇飞[1] 程安春[1] 汪铭书[1] 周伟光[1] 文明[1] 贾仁勇[1] 袁桂萍[1] 徐超[1] 韩晓英[1] 廖永洪[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国兽医学报》2005年第6期632-635,共4页Chinese Journal of Veterinary Science

基　　金：四川省杰出青年学科带头人基金后续资助项目(03ZQ026-28);四川省重点建设学科资助项目(SZD0418)

摘　　要：通过超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒(DEV)CH强毒株在鸭胚成纤维细胞(DEF)中的形态结构进行了研究.结果发现,DEV CH强毒株病毒核酸呈圆形颗粒状,直径35～45 nm,在胞核内常集中分布;病毒核衣壳呈圆形,直径90～100nm,在胞核和胞浆内都有分布;DEV核衣壳可根据所含核酸形态的差异分为空心核衣壳、内壁附有颗粒型核衣壳、同心圆形核衣壳和实心核衣壳;成熟的病毒粒子具有囊膜和皮层结构,呈圆形,直径150～300nm,存在于胞浆空泡内;DEV可在DEF中分别形成胞浆内和胞核内包涵体结构;伴随子代病毒在细胞内的出现,胞浆内还出现豆荚状、马蹄形、半圆形、圆形、同心圆形等与病毒发生有关的电子致密结构.Ultrathin sectioning and transmission electron microscopy of infected duck embryo fibroblasts（DEF） were employed to investigate the morphology of virulent duck enteritis virus（DEV） strain CH. The nucleic acid of DEV was round in shape with diameter of 35-45 nm and was often in a cluster in the nucleus of DEF. The nucleocapsid of DEV was round in shape with diameter of 90-100 nm and could be observed both in nucleus and cytoplasm of DEF. Based on their mophological characteristics,DEV nucleocapsids could be divided into 4 distinct groups which were the hollow nucleocapsid,the nucleocapsid with several nucleic acid particles closely attached to its inner wall ,the concentric circle nucleocapsid and the infarctate nucleocapsid. The mature DEV which had the structures of envelop and tegument was spherical in shape with diameter of 150-300 nm and located in cytoplasmic vacuoles. Intracytoplasmic inclusion bodies and intranuclear inclusion bodies could be observed respectively in the cytoplasm and nucleus of infected DEF. With the appearance of progeny DEV in DEF,some densely electron-stained virus-related structures which were rod-shaped ,U-shaped ,or of circle ,semicircle or concentric circle in appearance could be observed in the cytoplasm of DEF.

关 键 词：鸭病毒性肠炎病毒强毒 形态结构 鸭胚成纤雄细胞 

分 类 号：S852.65[农业科学—基础兽医学] S852.16[农业科学—兽医学]