兔膈肌肌纤维亚型和代谢酶活性对不同频率CES的适应性变化  

ADAPATATION OF MYOFIBRILLAE,MHC AND METABOLIC ENZYME OF RABBIT DIAPHRAGM MUSCLE TO DIFFERENT FREQUENCY CHRONIC ELECTRICAL STIMULATION

作　　者：张睢扬[1] 王方美[1] 刘刚[2] 王东林[2] 郭先健[2] 钱桂生[2] 

机构地区：[1]第二炮兵总医院呼吸科,北京100088 [2]第三军医大学新桥医院全军呼吸内科研究所,重庆400037

出　　处：《中国应用生理学杂志》2005年第4期367-370,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(39470325)

摘　　要：目的:探讨不同频率慢性电刺激对膈肌肌纤维亚型、肌球蛋白重链(MHC)亚型和代谢酶活性的适应性变化的影响。方法:分别用还原型辅酶Ⅰ四唑氮还原法、SDS-PAGE法和酶组织化学染色法观察、测定慢性电刺激后兔膈肌纤维类型、肌球蛋白重链(MHC)和NADHD等九种代谢酶活性变化。结果:①同对照组和慢性高频电刺激50Hz和100 Hz组比较,10 Hz和20 Hz慢性低频电刺激组膈肌Ⅰ型纤维,Ⅰ型MHC显著增加(P<0.01);ⅡB型纤维和ⅡB型MHC显著减少(P<0.01);慢性高频电刺激50Hz和100 Hz组则出现完全相反的变化(P<0.01)。②同对照组和慢性高频电刺激50 Hz和100 Hz组比较,慢性低频电刺激10 Hz和20 Hz组LDH和a-GPDHD活性明显降低(P<0.01),MDH、SDH、GDH、G-6-PD、NADHD和NADPHD活性显著升高(P<0.01);慢性高频电刺激50Hz和100 Hz组则出现完全相反的变化(P<0.01)。结论:肌纤维与代谢酶模式的适应性变化有明显的频率依赖性。Aim： To detect effect of the different frequency of chronic electrical stimulation（CES） on myofibrillar isoform, myosin heavy chain（MHC） and metabolic enzyme activities. Methods： The histochemical method and SDS-polyacrylamide gel electrophoresis were respectively employed. Results： ①There were a significant increase in Ⅰ myo-fibrillar isoform and Ⅰ MHC isoform and decrease in Ⅱ B myofibrillar isoform and Ⅱ B MHC isoforms in the chronic low frequency electrical stimulation（CLFES） 10 Hz and 20 Hz groups, but opposite results were found in the chronic high frequency electrical stimulation（CHFES） 50 Hz and 100 Hz groups. ②There were a significant increase in the aerobic-oxidative enzyme activities and capacity, and a concomitant significant drop in glycolysis enzyme activities in CLFES groups, but opposite results were found in CHFES 50Hz and 100Hz groups. Conclusion： It was suggested that there was a significant dependent relation between chronic electrical stimulational frequency and myofibrillae isoforms, myosin heavy chain（MHC） and metabolic enzyme activities.

关 键 词：膈肌 CES 肌纤维亚型 MHC 代谢酶 

分 类 号：Q445[生物学—生理学] R337.5[医药卫生—人体生理学]