葡萄籽中原花青素对实验动物主动脉收缩和血小板聚集的影响  

EFFECTS OF PROANTHOCYANIDINS ON CONTRACTILE ACTIVITY OF AORTIC SMOOTH MUSCLE AND PLATELET AGGREGATION IN EXPERIMENTAL ANIMALS

作　　者：张小郁[1] 李文广 郑天珍[1] 李伟[1] 

机构地区：[1]兰州大学基础医学院生理学教研室 [2]甘肃省中药新药临床前研究重点实验室,甘肃兰州730000

出　　处：《中国应用生理学杂志》2005年第4期383-386,共4页Chinese Journal of Applied Physiology

基　　金：甘肃省自然科学基金资助项目(ZS001-A23-059Y)

摘　　要：目的:研究葡萄籽中原花青素(PA)对大鼠离体主动脉平滑肌收缩活动和兔血小板聚集的影响。方法:采用大鼠离体主动脉环灌流方法,记录主动脉环张力变化,观察PA对去甲肾上腺素(NA)和KCl预收缩大鼠离体主动脉平滑肌收缩反应的舒张作用以及对NA量效曲线的影响。比浊法测定兔血小板聚集。结果:PA能明显抑制NA(10-6mol/L)预收缩大鼠离体主动脉环的反应,使NA量效曲线压低,最大反应降低,此作用无内皮依赖性,但对KCl预收缩主动脉环的舒张作用无明显影响,也不影响花生四烯酸(AA),ADP和胶原(collagen)蛋白诱导的兔血小板聚集。结论:PA能对抗NA而不影响KCl诱导的大鼠离体主动脉平滑肌的收缩,不影响兔血小板聚集。Aim： To study effects of proanthocyanidins（PA） on contractile activity of isolated aortic smooth muscle in rats and rabbit platelet aggregation. Methods： Isolated rat aortic muscle rings were adopted to observe the effects of PA on their contraction induced by noradrenaline（NA） or KCI, and their tensions were recorded by BL-310 experimental system of biological function. Rabbit platelet aggregation induced by arachidonic acid（AA）, adenosine diphosphate（ADP）, and collagen（Coil） was assayed by turbidimetry. Results： PA could significantly inhibit the contraction induced by NA（ 10^-6 mol/L）, low the concentration-response curves of NA and the maximal response on the endothelium-intact or endothelium-denuded aortic rings. But PA couldn＇t relax the aortic rings precontracted with KCl and had no influence on rabbit platelet aggregation induced by AA, ADP, and Coll. Conclusion： PA can inhibit the contraction induced by NA but not by KCI on isolated rat aortic rings. It also has no influence on rabbit platelet aggregation.

关 键 词：原花青素 主动脉平滑肌 去甲肾上腺素 血小板聚集 

分 类 号：R285.5[医药卫生—中药学]