人源性神经营养素-6对大鼠面运动神经元逆行溃变的保护作用  

PROTECTING EFFECT OF HUMAN-DERIVED NEUROTROPHIN-6 ON RETROGRADELY DEGENERATED MOTONEURONS OF FACIAL NUCLEUS OF RATS

作　　者：张承武[1] 李自成[1] 李丽[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,四川成都610041

出　　处：《中国应用生理学杂志》2005年第4期397-400,i0010,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助(39970279)

摘　　要：目的:探讨人源性神经营养素-6对受损伤神经元的保护作用,为全面了解人源性NT-6的神经生物学特性以及为临床上退行性神经病变的治疗提供实验依据。方法:将健康成年SD大鼠随机分为两组,即不做任何处理的正常对照组和切断一侧面神经后引起面运动神经元逆行溃变的实验组;实验组又根据面肌内注射物的不同分为空白对照组、人源性NT-6实验组和生理盐水对照组。动物饲养两周后,取脑干切片,行尼氏染色和胆碱乙酰基转移酶免疫组化染色,观察人源性NT-6对逆行溃变的面运动神经元的保护作用。结果:与空白对照组和生理盐水对照组相比,NT-6实验组面神经核尼氏染色的强度值和面神经核内ChAT阳性神经元数目显著增加。结论:人源性NT-6对由于轴突损伤引起逆行溃变的神经元具有保护作用。Aim： The protecting effect of human-derived neurotrophin-6 （NT-6） on injured neurons was investigated to test the neurobiological characteristics of human-derived NT-6. Methods.. Adult SD rats were used and divided into two groups, normal control group and experiment group. The right facial nerve of the experimental rats was sectioned. The experimental animals were subdivided into three groups, blank control group, NT-6 group and saline control group. The rats were raised for two weeks. Brain stem of the rats was removed and transversely diced. Nissle stain and ChAT immunohistochemieal stain of the slices were carried out to observe the role of NT-6 in protecting facial mortoneurons. Results ： The number of the ChAT positive neurons and the intensity of Nissle stain in the facial nucleus of NT-6 group were significantly augmented compared with that of the blank and saline control groups. Conclusion： The human-derived NT-6 could partially protect the facial motoneurons from retrograde degeneration induced by their axon damage.

关 键 词：神经营养素-6 运动神经元 逆行溃变 保护 

分 类 号：R338[医药卫生—人体生理学]