大鼠中脑黑质γ-氨基丁酸能神经元形态学增龄变化的定量研究  

Quantitative analysis of morphological changes of GABAergic neurons in substantia nigra of midbrain associated with increasine age in rats

作　　者：余资江[1] 余德立[2] 王景传[1] 许庭良[3] 姜俸蓉[3] 

机构地区：[1]贵阳医学院解剖学教研室,贵州省贵阳市550004 [2]贵阳中医学院第二附属医院五官科,贵州省贵阳市550001 [3]贵阳医学院组织胚胎学教研室,贵州省贵阳市550004

出　　处：《中国临床康复》2005年第41期84-85,i0003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨大鼠黑质γ-氨基丁酸神经元的增龄变化规律,为进一步揭示黑质病变的病因提供客观依据。方法:实验于2002-01／2003-07在贵阳医学院解剖学教研室完成。选择健康SD大鼠32只,分为一两个月龄、四五个月龄、十一二个月龄、≥24 个月龄4组,常规石蜡切片,中脑黑质连续冠状切片,进行苏木精-伊红染色、γ-氨基丁酸免疫组织化学染色,图像分析仪测量γ-氨基丁酸免疫阳性产物的吸光度和γ-氨基丁酸免疫反应阳性神经元的胞面积、体密度、数密度、圆球度,并对苏木精-伊红染色细胞进行计数。结果:32只大鼠均进入结果分析。4个月龄组大鼠随年龄增长,中脑黑质神经元数量减少,γ-氨基丁酸免疫反应阳性神经元平均吸光度减少 (P<0,001),γ-氨基丁酸免疫反应阳性神经元数密度、体密度、圆球度、平均体积均减少,平均截面积增加(P<0．01),呈增龄性变化。结论:黑质γ-氨基丁酸神经元随年龄增长而出现的衰老性变化,可能是黑质病变的原因之一。AIM： To study the regularities of GABAergic neurons in substantia nigra of midbrain with age increasing, so as to further provide objective evidence for revealing lesion of substantia nigra. METHODS： The experiment was carried out in the Department of Anatomy, Guiyang Medical College hctween January 2002 and July 2003. Thirty-two healthy SD rats were selected and divided into four groups： 1-2 months, 4-5 months,11-12 months and ≥ 24 months groups. Routine paraffin were made for hematoxylin and eosin （HE） staining and gamma-aminobutyric acid （GABA） immunohistochemical staining, the absorbance of GABA immune positive product and the area, body density, number density, globular degree of the GABA-positive neurons were measured with image analyzer, and the HE stained cells were counted. RESULTS： All the 32 rats were involved in the analysis of results. With the increasing age, the number of neurons in suhstantia nigra of midbrain in rats of 4 months old was decreased, the average absorbance of GABA- positive neurons was reduced （P 〈 0.001）, numher density, body density, globular degree, average volume of GABA-positive neurons were all reduced, but the average sectional area was increased （P〈 0.01）. CONCLUSION： The increased expression of GABA neurons in substantia nigra with aging may have important implication for the pathogenesis of substantia nigra disease in the region.

关 键 词：Γ氨基丁酸 神经元:黑质 大鼠 

分 类 号：R338.1[医药卫生—人体生理学]