硒化亚油酸与亚硒酸钠抗突变作用的比较研究  

COMPARATIVE STUDY ON ANTIMUTAGENESIS OF LINOLEIC ACID CONTAINING SELENIUM AND SODIUM SELENITE

作　　者：曹洁[1,2] 李瑞珍[1,2] 史勇亮[1,2] 吴端宗[1,2] 龚书明[1,2] 

机构地区：[1]第二军医大学卫生毒理学教研室 [2]第四军医大学军队卫生学教研室

出　　处：《癌变．畸变．突变》1996年第4期214-216,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文应用人全血白细胞非程序ＤＮＡ合成（ＵＤＳ）试验，对硒化亚油酸（Ｓｅ－ＬＡ）和亚硒酸钠（Ｎａ２ＳｅＯ３）两者抑制ＭＮＮＧ诱发ＤＮＡ损伤作用进行了观察和比较。结果表明：Ｓｅ－ＬＡ（０．０２３～３．４２４μｇ／ｍｌ）对ＭＮＮＧ诱发ＵＤＳ反应有明显抑制作用，其抑制率远大于含硒量相同的Ｎａ２ＳｅＯ３；０．０５μｇ／ｍｌＮａ２ＳｅＯ３（含Ｓｅ量为０．０２３μｇ／ｍｌ）对ＵＤＳ反应呈最强的抑制率（２１．７％），随Ｎａ２ＳｅＯ３浓度增高，其抑制作用反而降低，说明Ｎａ２ＳｅＯ３作为抗突变剂，它在高剂量时又呈现遗传毒性；当含Ｓｅ量≥０．０４６μｇ／ｍｌ时，Ｎａ２ＳｅＯ３对ＭＮＮＧ诱发ＤＮＡ损伤的抑制作用显著下降，而Ｓｅ－ＬＡ仍保持较高的抑制率（４２．２％～４３．９％）。The effects of linoleic acid containing selenium(Se-LA) and sodium selenite(Na 2SeO 3) were observed and compared on MNNG-induced DNA damaged with human whole blood cells by the test of unscheduled DNA synthesis(UDS).The results showed that (0.023￣3.424μg/ml) Se-LA significantly suppressed the responses of UDS induced by MNNG.Inhibitory effects of Se-LA were stronger than those of Na 2SeO 3 whose selenium concentration was the same as Se-LA.0.05μg/ml Na 2SeO 3 (containing 0.023μg/ml Se)expressed the largest inhibitory rate (21.7%) on UDS responses.With the concentration of Na 2SeO 3 increasing,the inhibitory effects reduced.The results revealed that Na 2SeO 3, as inhibitor of mutagenesis,could induce genetoxic effects by itself at higher dose levels.At the concentration of Se≥0.046μg/ml,although the inhibitory effects on MNNG-induced DNA damage decreased for Na 2SeO 3 ,Se-LA still maintained higher inhibitory rates(42.4￣43.9%).These results indicated that Se-LA significantly reduced the toxicity of selenium.

关 键 词：硒化亚油酸 亚硒酸钠 抗突变作用 

分 类 号：R979.1[医药卫生—药品]