荔枝核总黄酮的抗鸭乙型肝炎病毒作用  

Effect of total flavone of Litchi Chinensis Sonn on duck hepatitis B virus

作　　者：徐庆[1] 宋芸娟[2] 李丽亚[1] 侯巧燕[1] 

机构地区：[1]桂林医学院药理教研室,广西壮族自治区桂林市541004 [2]广西药用植物园,广西壮族自治区南宁市530023

出　　处：《世界华人消化杂志》2005年第17期2082-2085,共4页World Chinese Journal of Digestology

基　　金：广西科学研究与技术开发计划项目(桂科能);No.0330015-4C~~

摘　　要：目的:研究荔枝核总黄酮(TFL)抗鸭乙型肝炎病毒(DHBV)的作用.方法:采用桂林先天感染DHBV的麻鸭为动物模型,荔枝核总黄酮灌胃给药,2g/(kg·d)与1g/(kg·d),共15d,斑点杂交法观察用药前与用药后(1、5、10、15d)及停药后5d血清中DHBVDNA表达,HE染色观察肝脏组织病理学变化.结果:TFL2g/(kg·d)于用药后15d血清DHBVDNA水平为0.74±0.42,与生理盐水对照组1.64±0.68比较,明显降低(vs对照组,P<0.05(0/6),病理检查发现TFL大剂量组实验鸭肝细胞未发现明显的点灶坏死(0/6),与生理盐水对照组(4/6)比较有显著性差异(P<0.05).结论:TFL有抑制乙肝病毒的作用,并具有明显的抗炎、保肝作用.AIM： To study the anti-duck hepatitis B virus （DHBV） action of the total flavone of Litchi Chinensis Sonn （TFL）. METHODS： Guilin ducklings with congenital infection of DHBV were used as the animal modal. TFL was orally given to the ducks with the doses 2 g/（kg,d） and 1 g/（kgod） for 15 days. The expression of DHBV DNA was detected by spot blot hybridization before and after TFL was given. The pathological changes of the duck liver were also examined by HE staining. RESULTS： TFL at the dose of 2 g/（kg-d） can lower the level of serum DHBV DNA significantly （vs control group： 0.74±0.42 vs 1.64±0.68, P 〈0.05） 15 days after treatment. No marked necrosis was observed in the liver of high dose group （0/6）, but 4 of 6 cases appeared obvious necrosis in control group after treatment. There was significant difference between them （P 〈0.05）. CONCLUSION： TFL can protect duck liver against inflammation by the inhibition of DHBV.

关 键 词：荔枝核总黄酮 抗乙肝病毒 鸭乙肝模型 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]