转Endostatin基因角朊细胞移植对烧伤深Ⅱ°创面愈合及瘢痕增生的影响  

Effects of transplantation with ES gene transfected keratinocytes on the healing and cicatrize of deep partial-thickness burn wound

作　　者：梁晚益[1] 唐辉[1] 张琼[1] 刘旭盛[1] 黄跃生[1] 

机构地区：[1]第三军医大学附属西南医院烧伤研究所,重庆400038

出　　处：《中华实验外科杂志》2005年第12期1430-1432,i0019,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨转内皮生长抑制素(ES)基因角朊细胞移植对烧伤深Ⅱ°创面愈合及瘢痕增生的影响。方法将人体皮肤移植于裸鼠并造成深Ⅱ°烧伤创面。实验分为对照组(11只)、单纯角朊细胞移植组及转ES基因角朊细胞移植组(各10只)。对照组不行细胞移植,创面自行愈合;单纯角朊细胞移植组创面移植培养人角朊细胞;转基因移植组移植转ES基因角朊细胞。观察各组裸鼠创面愈合特点、瘢痕增生情况,并对愈合区组织进行病理切片检查、检测愈合区皮肤组织ES 蛋白表达、I、Ⅲ型前胶原含量。结果转基因移植组裸鼠创面愈合时间(13±5)d与单纯移植组 (14±5)d差异无统计学意义(P>0．05),但明显短于对照组[(25±7)d,P<0．01]。对照组裸鼠创面愈合后瘢痕增生明显,伤后100 d厚度≥0．22 cm,单纯移植组瘢痕增生厚度≥0．17 cm,转基因移植组仅有轻度瘢痕增生,愈合区皮肤组织ES蛋白检测阳性。单纯移植组、转基因移植组愈合区组织前胶原I含量(65．3±8．5)μg／g,(61．4±7．0)μg／g、前胶原I／前胶原Ⅲ比例(0．66±0．15,0．57± 0．13)明显低于对照组(1．51±0．37,P<0．01),而前胶原Ⅲ含量显著高于对照组(P<0．01)。结论转ES基因移植既可加速创面封闭,又能抑制愈合后瘢痕形成。Objective To investigate the effect of transplantation of keratinocytes transfected with endostatin gene on the wound healing of deep partial-thickness burn wound. Methods Normal human skin grafts was transplanted to the nude mice and a deep partial thickness burn wound was made after the survival of the skin grafts. The mice were divided into control group, simple keratinocyxes transplantation group （SKT） and endostatin gene transfected keratinocytes transplantation group （EGTKT）. The control group was free from transplantation and the wound was left to heal itself, while the SKT group was transplatlted with humatr keratinocytes. The characteristics of wound healing and scar hyperplasia of nude mice in all the groups were observed. Healing tissue was checked by pathological section, and the expression of endostatin and the content of procollagen （Type Ⅰ and Type Ⅲ） in the skin grafting site were detected. Results There was no remarkable difference in the healing time between the SKT group （ 14 ± 5） days and EGTKT group （ 13 ± 5） days, which was obviously shorter than in the control group [ （25 ± 7） days, P 〈 0.01]. The control group cicatrized significantly with a scar more than 0.22 cm thick 100 days after burn. The SKT group also had an obvious scar hyperplasia while the EGTKT group had a light scar formation with positive endostatin. The content of type Ⅰ procollagen and the value of type Ⅰ procollagen/type Ⅲ procoUagen in both SKT and EGTKT groups were significantly lower than those in the control group （P 〈 0.01 ）, while the content of type Ⅲ procollagen was markely higher than in control group （ P 〈 0.01 ）. Conclusion Transplantation with endostatln gene transfected keratinocytes can accelerate wound healing and inhibit scar hyperplasia.

关 键 词：角朊细胞 瘢痕 移植 创面愈合 细胞移植 烧伤 转Endostatin基因 

分 类 号：R644[医药卫生—外科学]