青藤碱对肾移植大鼠移植肾组织肿瘤坏死因子-α和CD80的影响  

Effects of Sinomenine on the expression of TNF-α and CD80 in rat renal allografts

作　　者：王毅[1] 宋珺[1] 姜黎明[1] 刘红军[1] 李建军[1] 王中华[1] 罗志刚[1] 

机构地区：[1]南华大学附属第二医院肾移植中心,湖南衡阳421001

出　　处：《中华实验外科杂志》2005年第12期1555-1556,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨青藤碱(SIN)对大鼠肾移植术后急性排斥反应免疫抑制作用的分子机制。方法实验动物模型随机分为:生理盐水(NS)组、SIN组、环孢素A(CsA)组、SIN+CsA组、对照组(V-Ⅶ),从每组中随机抽取半数,观察其存活时间,每日尿量及泌尿持续时间,另一半于术后第7天处死,检测血肌酐(Scr)尿素氮(BUN)水平,以及移植肾组织肿瘤坏死因子(TNF)-α、CD80 分子表达水平变化。结果 N．S组术后9 d内死;SIN+CsA组与NS、SIN、CsA组比较,差异存在统计学意义(P<0．05)。Scr、BUN:N．S组明显高于其他组,差异有统计学意义(P<0．05);各实验组与对照组比较差异有统计学意义,SIN+CsA组存在交互效应。外周血TNF-α表达水平、移植肾组织CD80表达:各实验组与对照组比较,差异有统计学意义(P<0．05),SIN+CsA组存在交互效应。结论 SIN可能通过下调移植肾组织TNF-α及细胞表面标记CD80分子的表达水平而产生免疫抑制作用。并与CsA存在协同作用。Objective To explore the molecular mechanism of immunosuppressive efficacy of alkaloid Sinomenine （SIN） against acute rejection following renal transplantation in rats. Methods The recipients were divided into 4 groups at random： NS group, SIN group, CsA group and SIN ＋ CsA group, The allograft survival time and 24 h urinary volume within 4 weeks were observed. Blood BUN and Cr levels were determined. The expression of TNF-α and CD80 in renal allografts was quantitatively detected by using immunohistochemical analysis. Results Recipients in NS group died within 9 days. Treatment with SIN or a subtherapeutic dose of CsA prolonged allograft survival only marginally. The combination of SIN and CsA had a statistically significant synergistic effect： Blood BUN and Cr levels were lower than other groups. SIN and C,sA significantly decreased the expressions of TNF-α and CD80. Conclusion SIN might play an immunosuppressive role in rat renal allograft models through down-regulating the levels of TNF-α and CD80. It has a significant synergistic effect with CsA.

关 键 词：青藤碱 肾移植 免疫抑制 TNF-α CD80 大鼠 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]