蛋白质-阳离子表面活性剂缔合微粒体系的光谱特性及分析应用  

The Spectral Feature of Protein-Cationic Surfactant Associated Particles and Its Analytical Application

作　　者：李振中[1] 蒋治良[1] 陈媛媛[1] 孙双娇[1] 周苏梅[1] 

机构地区：[1]广西师范大学资源与环境学系

出　　处：《应用化学》2005年第12期1304-1307,共4页Chinese Journal of Applied Chemistry

基　　金：广西自然科学基金(014403);广西高校百名中青年学年带头人计划资助项目

摘　　要：在NaOH-EDTA中,阳离子表面活性剂(Cationic surfactant,CS.如十四烷基二甲基苄基氯化铵,TDMBA)与蛋白质(Protein,Pro.如人血清白蛋白,HSA;牛血清白蛋白,BSA;γ-球蛋白,γ-G和卵白蛋白,Ova)可缔合形成稳定的[Pro-(TDMBA)n]m缔合微粒,从而导致共振散射和界面荧光增强. 它们最强共振散射峰均位于470 nm处,在340、400、420和520 nm处有4个共振散射峰. HSA缔合微粒体系用280 nm光激发时,在470 nm处产生1个较强的荧光峰,而在330 nm处蛋白质的荧光峰随着缔合微粒浓度的增大而猝灭. 在选定实验条件下, 0.16～8.0 mg/L HSA、0.08～16.0 mg/L BSA、0.16～16.0 mg/L γ-G和0.6～60.0 mg/L Ova分别与共振散射强度(ΔI470 nm)之间呈较好的线性关系,检出限(3σ)分别为0.01 mg/L HSA、0.01 mg/L BSA、0.01 mg/L γ-G和0.06 mg/L Ova. 该方法用于人血清试样中总蛋白的测定,结果令人满意.In the medium of NaOH-EDTA, cationic surfaetant tetradeeylmethylbenzylammonium chloride, TDMBA, associates with proteins, such as human serum albumin （HSA）, bovine serum albumin （BSA）, γ-globulin （γ-G） and ovalbumin （Ova）, to form associated particles, which enhances the resonance scattering. There were 5 resonance scattering peaks at 340, 400, 420, 470, and 520 nm, respectively, and the strongest one was at 470 nm after TDMBA was added to the HSA system. When the HSA association system was excited by the light at 280 nm, it exhibited a strong fluorescence peak at 470nm, while the fluorescence peak at 330 nm quenched with the addition of the associated particles. Under proper experimental conditions, the protein determination can be performed in the range of 0. 16 - 8.0 mg/L for HSA, 0.08 16.0 mg/L for BSA, 0. 16 - 16. 0 mg/L for T-G, and 0. 6 - 60. 0 mg/L for Ova. The detection limits are 0. 01, 0. 01, 0. 01, 0. 06 mg/L for HSA, BSA, γ-G, and Ova respectively. This method was applied to the determination of the total proteins in human serum with satisfactory results.

关 键 词：阳离子表面活性剂 HSA 缔合微粒 共振散射光谱 

分 类 号：O657.6[理学—分析化学]