离子束照射甘薯胚性细胞团获得突变体  

Mutants obtained from embryogenic cell suspension cultures irradiated by ion beam in sweet potato

作　　者：李爱贤[1] 刘庆昌[2] 

机构地区：[1]山东省农业科学院作物研究所,山东济南250100 [2]中国农业大学农学与生物技术学院,北京100094

出　　处：《山东农业科学》2005年第6期7-9,12,共4页Shandong Agricultural Sciences

基　　金：国家"863"计划项目(2002AA241031)资助

摘　　要：用0～100Gy12 C5+和0～200Gy4He2+分别照射甘薯品种栗子香的胚性悬浮细胞,用0～100Gy4He2+照射甘薯品种玉丰的胚性细胞团,以研究甘薯细胞水平离子束辐射的诱变效应.确定了甘薯胚性悬浮细胞适宜的12C5+和4He2+辐射剂量,分别为30～50Gy和70～100Gy;甘薯胚性细胞团适宜的4He2+辐射剂量为50～70Gy.得到大量再生植株,辐射后代的顶叶形、叶形、顶叶色、叶脉色、脉基色、顶端茸毛等性状出现了广泛的变异.Embryogenic cell suspension cultures of sweet potato variety Lizixiang were irradiated with ^12C^5＋ of 0～100Gy and 4He^2＋ of 0～200Gy respectively. Based on the survival cell aggregates and the regeneration plants,30～50Gy and 70～100Gy were determined as the optimal dose of ^12C^5＋ and ^4He^2＋ respectively. Embryogenic cell aggregates of sweet potato variety Tamayutaka were irradiated with ^4 He^2＋ of 0～100Gy. It was indicated that the optimal dose of ^4He^2＋ was 50～70Gy. Lots of regen- eration plants were obtained from the irradiated embryogenic cultures.

关 键 词：甘薯 胚性细胞团 离子束照射 突变体 

分 类 号：S531.035.2[农业科学—作物学]