雷琼牛mtDNA D—loop遗传多态性研究  

Study on mtDNA D-loop Genetic Diversity in Leiqiong Cattle

作　　者：王朝锋[1] 雷初朝[1] 陈宏[1] 蔡欣[1] 党瑞华[1] 欧江涛 

机构地区：[1]西北农林科技大学动物科技学院 [2]海南农科院畜牧兽医研究所,海南海口571100

出　　处：《黄牛杂志》2005年第5期14-15,共2页Journal of Yellow Cattle Science

基　　金：国家自然科学基金(30471238);西北农林科技大学拔尖人才支持计划;863项目(2003AA2430051)资助

摘　　要：对雷琼牛6个个体的mtDNA D—loop区910bp进行分析，检测到2种单倍型，其核苷酸多态位点5个，约占所测核苷酸总长的0．55％，其中有2个转换，2个颠换，1个插入/缺失。雷琼牛mtDNA D—loop区核苷酸多样度(π值)为0．15％，单倍型多样度(H)为0．33，表明雷琼牛mtDNA遗传多样性贫乏。经比较分析，雷琼牛起源于瘤牛。The complete mitochondrial D-loop sequences, 910 bp in length, in 6 Leiqiong cattle were analyzed. The results revealed 2 mitochondrial haplotypes, 5 polymorphic sites, accounting for 0. 55% of 910 bp sequences. Among these polymorphic sites, there were 2 transitions, 2 transversions and 1 insertion/ deletion. The nucleotide diversity （π value） and haplotype diversity （H） estimated from mtDNA D-loop region in Leiqiong cattle breed were 0. 15 % and 0. 33, respectively, showing that there was poor mitochondrial genetic diversity in Leiqiong cattle population. Comparison to the Bos indicus mtDNA D-loop indicated that the origin of Leiqiong cattle was from Bos indicus.

关 键 词：雷琼牛 MTDNA D-LOOP 多态性 单倍型 起源 

分 类 号：S823.2[农业科学—畜牧学]