激光诱导榆树单倍体植株及无融合生殖的研究  

Studies on Elm Haploid Apomixis Induced by Laser

作　　者：陈震古[1] 刘小刚[1] 许成林[1] 余皖苏[1] 訾兴中[1] 李梦元[1] 

机构地区：[1]安徽农业大学生物技术研究中心激光研究室,安徽省滁州市琅琊山森林公园管理处,安徽农业大学

出　　处：《量子电子学》1996年第4期391-396,共6页

摘　　要：试验应用激光诱导单倍体无融合生殖，主要是通过对琅琊榆（ＵｌｍｕｓｃｈｅｎｍｏｕｉｉＣｈｅｎｇ）、醉翁榆（Ｕ．ｇａｕｓｓｅｎｉｉＣｈｅｎｇ）花药、子房离体组织培养，得出促使出愈率最高并获得器官分化的是Ｈｅ－Ｎｅ激光照射剂量２０Ｊ／ｃｍ２．其中醉翁榆子房愈伤组织经过长达５２０天的分化培养，还能从它的次生愈伤组织中得到绿芽分化。试验还获得琅琊榆人工去雄后用Ｈｅ－Ｎｅ激光、ＣＯ２激光不同照射剂量诱导无融合生殖产生的种子。重复试验发现，经激光处理的去雄花枝一直不发叶，而与ｃｋ形成了鲜明对比。The haploid apomixis was induced by laser in culturing the anthers and ovaries of Ulmus Chenmouii and U,gaussenii in vitro in the experiment.The results showed that the irradiative dosage of He-Ne laser with 20 J/cm2 could cause the highest rate of callus and organ differentiation.Even after being cultured for 520 days,the ovary secondary callus of U.gaussenii was differentiated to be green buds.The apomixis seeds were gained from U.Chenmouii inflore-scence with artificial emasculation induced by He-Ne and CO2 laser with different irradiative dosages.The repetition of the experiment shows that the branches treated by laser havn't grown leaves all along, which were emasculated artificially in advance, and formed a sharp contrast with the control.

关 键 词：榆树 激光诱变 诱变 醉翁榆 单倍体 无融合生殖 

分 类 号：S792.19[农业科学—林木遗传育种] S722.35[农业科学—林学]