基于罗丹明S缔合微粒荧光猝灭效应测定ClO^-  

A Method for the Determination of Trace Hypochlorite Based on the Fluorescence Quenching Effect of the Rhodamine S Particles

作　　者：章表明[1] 蒋治良[1] 

机构地区：[1]广西师范大学资源与环境学系,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2005年第4期69-72,共4页Journal of Guangxi Normal University:Natural Science Edition

基　　金：广西自然科学基金资助项目(014403);广西高校百名中青年学科带头人计划资助项目

摘　　要：在稀盐酸介质中,C lO-与过量的I-反应生成I3-,I3-与罗丹明S(R hS)、罗丹明B(R hB)及丁基罗丹明B(b-R hB)形成缔合微粒而导致各体系的荧光分别在550,580,580 nm处发生猝灭.C lO-浓度分别在0.020~0.35,0.020~0.51,0.020~0.35 m g/L范围内与各体系的荧光猝灭强度成线性关系.各体系的检测限分别为0.016,0.028,0.029 m g/L.据此建立了测定次氯酸根的荧光猝灭分析法.其中C lO--K I-R hS体系的灵敏度高而且稳定性较好,用于漂渍液和漂白粉中C lO-的测定,并与碘量法对照,结果满意.In the medium of hydrochloric acid,ClO^- reacts with excessive I^- to form I3^-. It combines respectively Rhodamine S （RhS）,Rhodamine B （RhB） and butyl-Rhodamine B （b-RhB） to form association particles ,which result in fluorescence quenching of the RhS,RhB and b-RhB systems at 550,580 and 580 nm, respectively. The fluorescence quenching is proportional to the concentration of ClO^- in the range of 0. 020-0.35,0. 020-0.51,0. 020-0.35 mg/L and the detect limits for each system are 0. 016, 0. 028 and 0. 029 mg/L respectively. Based on these facts,a fluorescence quenching methods for determination of trace ClO^- have been proposed. Owing to the system of RhS of high sensitivity and good stability,it has been applied to the determination of ClO^- in bleaching powder and liquid,with satisfactory resuits.

关 键 词：次氯酸根 荧光猝灭法 罗丹明S 

分 类 号：O657[理学—分析化学]