植物病原棒形细菌的RAPD分析  

RAPD analysis of plant pathogenic coryneform bacteria

作　　者：尹燕妮[1] 陈永芳[1] 李师默[1] 郭坚华[1] 

机构地区：[1]南京农业大学植物病理学系,南京210095

出　　处：《微生物学报》2005年第6期837-841,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(39970481);国家"863计划"(2003AA249020)~~

摘　　要：采用RAPD分析技术对萎蔫短小杆菌落葵致病变种(Curtobacterium flaccumfaciens pv.basellae pv.nov.)和萎蔫短小杆菌糖甜菜致病变种(Curtobacterium flaccumfaciens pv.beticola pv.nov.)及植物病原棒形细菌4个属的15个菌株进行分类研究:从50条随机引物中筛选出20条,共产生225条带,多态性条带占80.4%。遗传相似矩阵及UPGMA聚类分析,表明这两个新致病变种与萎蔫短小杆菌属亲缘关系近,最小相似系数为0.6511;与其他属细菌亲缘关系较远;结合前人研究结果对植物病原棒形细菌新近提出的分类地位的改变进行了探讨。RAPD analysis classification of two new was used for the taxonomy of plant pathogenic coryneform pathogens （Curtobacterium flaccumfaciens pv. basellae pv bacteria, especially for the nov. and Curtobacterium flaccumfaciens pv. beticola pv. nov. ）. 20 random primers were screened from 50 ones to detect polymorphism among the total strains used. 80.4% were polymorphic bands among the 225 ones produced. The results of pairwise similarity and UPGMA cluster analysis suggest that the two new pathovars of sugar beet （ Beta vulgaris var. saccharifera） and malabar spinach （BaseUa rubra ） are genetically close related with Curtobacterium flacumfaciens, and the minimal similarity coefficient is 0.6511. According to the RAPD analysis and previous research, some newly made taxonomic changes of the plant pathogenic coryneform bacteria are discussed.

关 键 词：RAPD 分类 萎蔫短小杆菌 植物病原棒形细菌 

分 类 号：S432.42[农业科学—植物病理学]