以四氢嘧啶为主要相容性溶质的中度嗜盐菌I15的分离和特性研究  

Isolation and characteristic of a moderately halophilic bacterium accumulated ectoine as main compatible solute

作　　者：何健[1] 汪婷[1] 孙纪全[1] 顾立锋[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《微生物学报》2005年第6期900-904,共5页Acta Microbiologica Sinica

基　　金：国家自然科技资源平台项目(2004DKA30560_2)~~

摘　　要：从草地土壤中分离到一株中度嗜盐菌I15,经过16SrDNA(GenBank登录号为DQ010162)序列分析、形态学和生理生化特征分析,该菌株初步鉴定为Virgibacillusmarismortui。I15能在0%～25%NaCl的培养基中生长,最适生长NaCl浓度为10%,最适生长温度为30℃,最适pH为7.5～8.0。在高盐条件下,I15细胞内主要的相容性溶质为四氢嘧啶,在15%NaCl培养基中其含量达到1.608mmol(g·cdw),占到相容性溶质总摩尔含量的89.6%。渗透冲击试验表明I15细胞内四氢嘧啶在低渗冲击时能够快速分泌到细胞外,在高渗冲击冲剂时能够较快地重新合成。A moderately halophilic bacterium（designated strain I15）was isolated from lawn soil. Based on the analysis of 16S rDNA （GenBank accession number DQ010162）, morphology, physiological and biochemical characteristics, strain I15 was identified as Virgibacillus marismortuii. This strain was capable of growing under 0% - 25% NaCl, and exhibited an optimum NaCl concentration of 10% and an optimum temperature of 30℃ and an optimum pH of 7.5 - 8.0 for its growth, respectively. Under hyperosmotic stress, strain I15 accumulated ectoine as the main compatible solute. Under 15 % NaCl conditions the intracellar ectoine can reach to 1. 608mmol/（g· cdw）, accounted for 89.6 % of the total compatible solutes. The biosynthesis of ectoine was under the control of osmotic, and the accumulated ectoine synthesized intraceilularly can released under hypoosmotic shocks and resynthesis under hyperosmotic shock rapidly.

关 键 词：中度嗜盐菌 相容性溶质 四氢嘧啶 细菌泌乳工艺 

分 类 号：Q939.9[生物学—微生物学]