螺旋藻复方有效部位配方对慢性运动性疲劳大鼠脑组织基因表达谱的影响  

Effect of Spirulina's Complex Prescriptions(SACP)Available Composition Combination (SACP'ACC) on Gene Expression Profile in Brain of Rats with Chronic Exercise Fatigue

作　　者：朱梅菊[1] 谢振良[1] 

机构地区：[1]湛江师范学院体育科学学院,广东湛江524048

出　　处：《体育科学》2005年第12期61-64,共4页China Sport Science

基　　金：广东省自然科学基金资助项目(31938);湛江市科技招标项目(湛科[2005]97号)。

摘　　要：目的:研究螺旋藻复方有效部位配方对慢性运动性疲劳大鼠脑组织基因表达谱的影响,探索螺旋藻复方有效部位配方抗疲劳的分子药理作用机制。方法:分别从慢性运动性疲劳模型组、螺旋藻复方有效部位配方治疗组SD大鼠的脑组织中抽提总RNA,Cy3、Cy5荧光标记,反转录分别合成cDNA探针后,与含有4096个大鼠基因的BiostarR20S基因表达谱芯片杂交,Genepix4000B扫描仪扫描,GenePixPro3.0软件分析表达信号。结果:螺旋藻复方有效部位配方治疗后差异表达的基因有98个,其中,已知基因88个,未知基因10个,已知基因中有12个基因上调,76个基因下调。结论:螺旋藻复方有效部位配方对慢性运动性疲劳大鼠脑组织基因表达具有多方面的调控作用,从分子水平阐释了螺旋藻复方有效部位配方抗中枢疲劳的药理作用机制。This paper studies the pharmacologic mechanism of SACP＇ACC in antagonizing chronic exercise fatigue,explores its＇ effect on gene expression profile in brain of rats with chronic exercise fatigue. Total RNAs are isolated from rats with chronic exercise fatigue. The mRNAs are reversal transcribed to cDNA with incorporation of fluorescent Cy3 dUTP to prepare hybridization probes. The PCR products of 4096 genes are spotted on the chip after a serial treatment. The mixed probes are hybridized to the cDNA microarray. Genepix 4000B and Gene Pix Pro 3. 0 software are used to scan and analyze the fluorescent signals. The result shows that in the group treated with SACP＇ACC, 98 genes have expression profiles difference to that of in the model group in brain tissue ,in which 88 genes are known, 10 genes are unknown. In known genes, 12 are regulated up and 76 down. It concludes that SACP＇ACC has regulatory effect on gene exression in rats with chronic exercise fatigue, which elucidates part of pharmacologic mechanism of molecular level.

关 键 词：螺旋藻 运动性疲劳 基因表达谱 鼠 动物实验 

分 类 号：G804.5[文化科学—运动人体科学]