mecA基因PCR扩增法检测耐甲氧西林金黄色葡萄球菌  

PCR of mecA gene for detection of methicillin resistant Staphylococcus aureus

作　　者：谢闺娥[1] 徐霞[1] 杨能[1] 

机构地区：[1]广州医学院检验系,广东广州510182

出　　处：《中国微生态学杂志》2005年第6期438-438,441,共2页Chinese Journal of Microecology

基　　金：广州市医药卫生科技项目(2005-YB-135);广州医学院基金(03-K-32)

摘　　要：目的应用m ecA基因PCR扩增法检测耐甲氧西林金黄色葡萄球菌(m eth ic illin res istan tstaphy lococcus aureus,M RSA)。方法临床分离的70株金黄色葡萄球菌,应用m ecA基因PCR扩增法鉴定M RSA,并与苯唑西林纸片扩散法进行比较。结果70株金黄色葡萄球菌用PCR扩增法和纸片扩散法有6株鉴定有差异,4株m ecA基因阳性而纸片扩散法鉴定为敏感,1株m ecA基因阳性纸片扩散法鉴定为临界耐药,1株m ecA基因阴性却表现为苯唑西林耐药,2种方法符合率为91.43%。结论m ecA基因PCR扩增法可以准确、快速判定M RSA,特别是对隐匿型或低水平耐药菌株的检出有重要的价值。Objective To establish a method of polymerase chain reaction（PCR） of mecA gene for detection of methicillin resistant Staphylococcus aureus （MRSA）. Methods A total of 70 strains of Staphylococcus aureus were isolated from the hospital. MecA PCR assay for detecting MRSA was compared with oxacillin disk diffusion method. Results Among the 70 Staphylococcus aureus isolates,there were four strains positive for mecA gene by PCR,while oxacillin susceptible by disk diffusion. One strain positive for mecA gene showed borderline resistance. One isolate negative for mecA gene was found to be oxacillin resistant. The consistence of the two methods was 91.43%. Conclusion Detection of mecA gene by PCR was a rapid and efficient approach to identify MRSA,especially to the strains with ambiguous and low-level oxacillin resistance.

关 键 词：MECA基因 耐甲氧西林金黄色葡萄球菌 PCR 

分 类 号：R378[医药卫生—病原生物学]