聚合酶链反应快速诊断疱疹病毒性脑炎  

Application of polymerase chain reaction in the rapid diagnosis of herpesvirus encephalitis

作　　者：卓礼梅[1,2] 徐帆[3] 陈火胜[2] 郭辉玉[2] 

机构地区：[1]广州,510089 [2]中山医科大学微生物学教研室 [3]广州中山大学昆虫所

出　　处：《中华实验和临床病毒学杂志》1996年第2期174-176,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：选择疱疹病毒科病毒 DNA 多聚酶基因高保守区中的1对引物,1次聚合酶链式反应可同时扩增疱疹病毒科的4种病毒(HSV1,HSV2,EBV,HCMV)相应的 DNA 片段。根据扩增产物的分子量大小及对扩增产物酶切分析,可将标本中的4种病毒准确分型。用该法检测临床疑为病毒性脑炎患者和其他中枢神经系统疾病患者(对照组)的脑脊液(CSF)。脑炎组 CSF 阳性率30%(9/30),对照组 CSF 阳性率6.7%(2/30);其中8例为 HSV1阳性,2例为 HCMV 阳性,1例为 HSV2阳性。本法最早可在发病第3d 的病人 CSF 中检出 HSV1-DNA,检测敏感性达10fg HSV1-DNA。A single pair of oligonucleotide primers selected within a highly conserved region of the DNA poly- merase gene of the herpesvirus was designed to amplify related viral genomes,i.e.herpes simplex virus type.1,her- pes simplex virus type 2.Epstein-Barr virus,and cytomegalovirus,by the polymerase chain reaction.According to the molecular weight of the amplified products and a restriction enzyme analysis of these amplified products allowed accurate characterization of the herpesvirus type.In the assay,cerebrospinal fluids(CSF)from 30 patients with acute encephalitis and 30 patients with other central nervous system infections as controls were applied.The positive rate of CSF from encephalitits group was 30%,and the positive rate of control group was 6.7%.8 samples of CSF were posi- tive for HSV1-DNA,2 for HCMV-DNA and 1 for HSV2-DNA.The HSV1-DNA in patient's CSF can be detected by this method as early as 3 days after onset of neurological illness,with sensitivity reaching the level of 10fg HSV1- DNA.Our results suggest that PCR may be a specific,sensitive and rapid technique for the early diagnosis of her- pesvirus encephalitis.

关 键 词：脑炎 病毒学 聚合酶链反应 疱疹病毒 诊断 

分 类 号：R512.304[医药卫生—内科学]