猪胸膜肺炎放线杆菌山东分离株的血清型与Apx毒素型研究  

Study on Serotype and Apx Toxins Type of Actinobacillus pleuropneumoniae Strains Isolated from Shandong Province

作　　者：刁有祥[1] 丁家波[1] 姜世金[1] 陈本龙[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《中国农业科学》2005年第11期2349-2354,共6页Scientia Agricultura Sinica

基　　金：山东省重大畜禽疫病综合防治技术项目(011020102)资助

摘　　要：从山东省不同地区采集的85份具有严重呼吸道症状病死猪的肺脏、气管和扁桃体进行胸膜肺炎放线杆菌(APP)的分离鉴定,采用凝集试验和PCR方法对分离菌株进行血清型和APX毒素型的检测.结果从山东省不同地区分离到20株分属5个血清型的APP,其中,血清7型6株、血清5型5株、血清3型4株、血清4型3株、血清8型2株,血清7型、5型为优势血清型.不同地区分离的APP血清型不同,同一地区也存在不同的血清型.PCR检测结果表明,不同血清型的APP共含有4种溶血毒素(Apx),其中,血清3、4、8型APP含ApxⅡ、ApxⅢ和ApxⅣ,血清5型含Apx Ⅰ、ApxⅡ和ApxⅣ,血清7型含ApxⅡ和ApxⅣ,20株APP分离株均含有ApxⅡ和ApxⅣ两种毒素,表明野毒株的血清型与毒素基因的种类有一定的相关性.毒素序列分析结果表明,相同毒素型APP,其毒素基因序列的同源性在99.5%～100%之间,与血清型无关.致病性试验结果表明,所含毒素类型不同的APP对小白鼠的致病性不同,其中含Apx Ⅰ和ApxⅡ的血清5型APP毒力最强.该研究为猪传染性胸膜肺炎的免疫预防、亚单位疫苗的研制及基于ApxⅣ的PCR检测奠定了基础.Twenty field strains of Actinobacillus pleuropneumoniae （APP） were isolated from lung, tonsils and trachea in 85 pigs with sever pleuropneumonia in different districts of Shandong Province. Slide agglutination test and PCR were used to determine their serotype and Apx toxins type, respectively. The results of slideagglutination tes.t indicated that there were 5 serotypes among the 20 field strains, serotype 7（6/20） and serotype 5 （5/20） were the dominant, the other serotypes were serotype 3（4/20）, serotype 4（3/20） and serotype 8（2/20）. The PCR results indicated that there were four kinds of Apx toxins in the 20 field strains, among them, serotype 3, 4, 8 contain Apx Ⅱ, ApxⅢ and ApxⅣ, serotype 5 contains Apx Ⅰ, Apx Ⅱ and ApxⅣ, serotype 7 contains Apx Ⅱ and ApxⅣ, Apx Ⅱ and ApxⅣ existed in all the 20 field strains. The results of Apx sequence analysis indicated that the homogeneity of Apx gene was 99.5%- 100% in different serotype APP if they had the same type of Apx. The pathogenicity was different when mice inoculated with APP which contain different Apx, serotype 5 APP was very virulent, for it contains ApxⅠ and Apx Ⅱ. This study has established the foundation for control Porcine infectious pleuropneumonia in immune prevention, subunit vaccine and PCR diagnosis based on ApxⅣ gene.

关 键 词：猪传染性胸膜肺炎 胸膜肺炎放线杆菌 血清型 毒素型 

分 类 号：S852.61[农业科学—基础兽医学]