Prospect of creating trans-genic animals by using sper-matogonial transplantation  

作　　者：BOU Shorgan 

机构地区：[1]The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, China

出　　处：《Chinese Science Bulletin》2005年第22期2537-2545,共9页

基　　金：supported by the Special Invited Professor Research Starting Fund of Inner Mongolia University(Grant No.203059);Important Projects of Natura1 Science Fund of Inner M ongolia Municipality(Grant No.200408020402);he Sub-Projects of National Hjgh-Tech D&P Program(863 Program)(Grant Nos.2005AA206110&200AA242061).

摘　　要：Transgenic animal technology is a powerful tool for researching bioscience, biomedicine, bioreactor, and ag- riculture. There are various ways to produce transgenic animals. The most common ways currently available are pronuclear microinjection and nuclear transfer techniques. However, these methods usually result in low efficiency, causing mosaic (in pronuclear microinjection), or develop- mental abnormalities (in nuclear transfer). In 1994, Brinster and his colleagues reported an original method to transfer spermatogonial stem cells from donor to recipient mice. The donor spermatogonia were able to form spermatozoa in re- cipient testes, and to produce progeny carrying the donor’s genetic characters. Since then, a series of novel methods were invented by using spermatogonia transplantation. These new methods facilitate the research and application of sper- matogonia. Some of these methods, when combining with genetic modification methods, will form a novel methodology for creating transgenic animals. The present paper reviews the achievements of research on spermatogonia transplanta- tion related to creating transgenic animal. Such as, trans- plantation techniques, cryopreservation of spermatogonia, preparation of recipients, long-term proliferation of sper- matogonia in culture, genetic modification of spermatogonia, and characterization of germ line transmission of the modi- fied gene, etc. Furthermore the methodologies for creating transgenic animals by using spermatogonia transplantation were described. Based on the difference between donors and recipients used, the methodology is categorized into two groups: allogeneic transplantation, and autologous trans- plantation. Although progress in this research area has been swift, potential difficulties remain to be overcome in each approach. The advantages and existing problems in the methodology are discussed.Transgenic animal technology is a powerful tool for researching bioscience, biomedicine, bioreactor, and agriculture. There are various ways to produce transgenic animals. The most common ways currently available are pronuclear microinjection and nuclear transfer techniques. However, these methods usually result in low efficiency, causing mosaic （in pronuclear microinjection）, or developmental abnormalities （in nuclear transfer）. In 1994, Brinster and his colleagues reported an original method to transfer spermatogonial stem cells from donor to recipient mice. The donor spermatogonia were able to form spermatozoa in recipient testes, and to produce progeny carrying the donor＇s genetic characters. Since then, a series of novel methods were invented by using spermatogonia transplantation. These new methods facilitate the research and application of spermatogonia. Some of these methods, when combining with genetic modification methods, will form a novel methodology for creating transgenic animals. The present paper reviews the achievements of research on spermatogonia transplantation related to creating transgenic animal. Such as, transplantation techniques, cryopreservation of spermatogonia, preparation of recipients, long-term proliferation of spermatogonia in culture, genetic modification of spermatogonia, and characterization of germ line transmission of the modified gene, etc. Furthermore the methodologies for creating transgenic animals by using spermatogonia transplantation were described. Based on the difference between donors and recipients used, the methodology is categorized into two groups： allogeneic transplantation, and autologous transplantation. Although progress in this research area has been swift, potential difficulties remain to be overcome in each approach. The advantages and existing problems in the methodology are discussed.

关 键 词：生物科学 动物学 生物医学 显微注射 遗传基因 

分 类 号：Q789-1[生物学—分子生物学]