大鼠严重烧伤后肠绒毛的改变  

Morphological changes in intestinal villi after severe burns in rats

作　　者：荣新洲[1] 张涛[1] 杨荣华[1] 黄晓桦[1] 李庆辉[1] 

机构地区：[1]广州医学院广州市第一人民医院烧伤科,510180

出　　处：《中华烧伤杂志》2005年第6期459-461,共3页Chinese Journal of Burns

基　　金：广州市科技计划资助项目(2003Z3-E0371)

摘　　要：目的观察大鼠烧伤后肠绒毛的改变,探讨其与发生肠源性感染的可能关系。方法选用Wistar大鼠50只,其中10只作为对照组,不作任何处理;余下40只作为烧伤组,造成30%TBSAⅢ度烫伤(以下称烧伤)后,经腹腔补充等渗盐水4ml/100g.观察对照组及烧伤组大鼠伤后8、12、24、48h回肠末端肠黏膜形态、中央乳糜管管径、小肠含水量百分比的变化。结果对照组大鼠肠绒毛形态正常;烧伤组伤后各时相点肠绒毛肿胀,肠黏膜细胞间出现宽大裂隙,局部有绒毛缺损。烧伤组大鼠伤后各时相点中央乳糜管持续扩大,其管径均明显大于对照组(P<0.01),同时管中存有大量的淋巴液。烧伤组大鼠伤后8、12h小肠含水量百分比分别为(70.5±2.2)%、(69.5±3.1)%,明显低于对照组(76.9±1.5)%(P<0.01),而伤后24、48h则与对照组相近(P>0.05).结论严重烧伤后大鼠肠绒毛的变化可能会促进肠道毒素和细菌的侵入,肠淋巴通道可能是肠源性感染的重要途径。烧伤后早期肠黏膜对肠道水分的回吸收过程是短暂加强的。Objective To investigate the morphological changes in intestinal villi after severe burns in rats, so as In explore its possihle relationship with enteral bacterial translocation. Methods Fifty Wistar rats were employed in the study, 10 of them were assigned to the control group （C）. The rest 40 rats were subjected to 30% TBSA full-thickness scalding（ burn group,B）. 4ml/100g normal saline was given intraperitoneally to the injured rats, The changes of the caliber of the central chvliferous vessel, the intestinal water content and the mucosal morphology of the terminal ileum were determined in the rats in C group and in B group at 8,12, 24 and 48 postburn hours. The morphology of villi was observed with scanning electron microscope and light microscope. Results The ileal villi appeared normal in C group. The central ehvliferous vessel dilated persistently, in rats of B group at all postburn time points, and dilatation was more evident in B group compared with control group （ P 〈0.01）,At the same time an abundant amount of lymph was ohserved in the central chvliferous vessel. The intestinal water contents decreased to （ 70.5 ± 2.2）% and （69.5 ±3. 1 ）% in rats of B group at 8 and 12 PBHs, respectively,, and they were obviously lower than that in C group （76.9±1.5）% ,（ P 〈0.01）.The intestinal water content in B group was similar to that in C group at 24 and 48 PBH （ P 〉 0. 05 ）. Conclusion The morphological changes in the intestinal villi of rats with severe hurn injury may predispose the invasion of enteral toxin and bacteria, Intestinal lymphatics can be an inlpnrtant route for enteral bacterial translocation. The water reabsorption of the intestinal mueosa can be transiently enhanced during early posthurn stage.

关 键 词：烧伤 肠黏膜 感染 中央乳糜管 

分 类 号：R644[医药卫生—外科学] R364.14[医药卫生—临床医学]