急性放射性皮肤溃疡中血小板源性生长因子及其受体的表达  

The expression of platelet-derived growth factor-A and its receptor in acute radiation-induced skin ulcer in rats

作　　者：曹卫红[1] 柴家科[1] 杨志祥[2] 王德文[2] 盛志勇[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100037 [2]军事医学科学院放射医学研究所

出　　处：《中华烧伤杂志》2005年第5期359-362,共4页Chinese Journal of Burns

摘　　要：目的观察不同创面血小板源性生长因子(PDGF)A及其受体(PDGFR)α的动态表达,探讨急性放射性皮肤溃疡难愈合的机制.方法应用γ射线单次照射Wistar大鼠,制作急性放射性皮肤溃疡模型(照射组,55只),以皮肤全层切割伤模型大鼠作为创伤对照(创伤组,55只),另取5只正常大鼠作为对照组.采用免疫组织化学及原位逆转录-聚合酶链反应(RT-PCR)等方法,观察不同时相点各组大鼠创面内PDGF-A、PDGFR-α和PDGF-A mRNA的表达. 结果对照组大鼠皮肤中PDGF-A及PDGFR-α表达为阴性;创伤组大鼠炎症反应期及肉芽组织期(伤后3～9 d)PDGF-A的积分吸光度(IA)值为20.0±1.6、28.3±1.0;照射组炎症反应期及肉芽组织期(伤后14～28 d)其PDGF-A的IA值各为14.0±1.2、20.3±1.2,PDGF-A及PDGFR-α和PDGF-A mRNA的表达较创伤组明显减弱,至瘢痕形成期(伤后55 d)时表达进一步减弱.结论急性放射性皮肤溃疡内PDGF-A及PDGFR-α的表达减弱,可能是创面难愈合的机制之一.Objective To investigate the dynamic expression of platelet-derived growth factor-A （PDGF-A） and its receptor alpha （PDGFR-α） in different acute radiation-induced skin ulcers, and to explore the underlying mechanism involved in retarded healing of the ulcer. Methods The model of acute radiation-induced skin ulcers in rats was replicated with 50 Gy ^60Co γ rays to the skin （ radiation group, R, n = 55） , rats with full -thickness skin excision wounds as control group （T, n = 55 ） , and 5 normal rats to serve as normal control （NC） group. The expression of PDGF-A and PDGFR-α protein and PDGF-A mRNA was respectively assessed by means of histochemistry and in situ RT-PCR. Results No PDGF-A expression was identified in the rat skin in NC group. The expression of PDGF-A and PDGFR were reduced in R group during inflammatory responsive and granulation formation periods （14 - 28 days after radiation, the IA value of PDGF-A varied from 14.0 ± 1.2 to 20.3 ± 1.2 compared with that in T group in which the IA value of PDGF-A at the same period （3 - 9 days after injury） varied from 20.0 ± 1.6 to 28.3 ± 1. 0, and reduced gradually during scar formation period （55 days after radiation）. Conclusion The reduction of PDGF-A and PDGFR-expression may be partially involved in the mechanism of retarded healing of acute radiation-induced skin ulcers.

关 键 词：血小板源性生长因子 受体 血小板源性生长因子α 辐射损伤 溃疡 伤口 愈合 

分 类 号：R818[医药卫生—放射医学]