维拉帕米抑制大鼠心脏异位移植术后冠状血管内膜增厚  

Verapamil inhibiting vasculopathy after heart transplant in rats

作　　者：肖永光[1] 黄杰[1] 毛志福[1] 程邦昌[1] 蔡享道[1] 

机构地区：[1]武汉大学人民医院胸心外科,430060

出　　处：《中华器官移植杂志》2005年第12期726-727,共2页Chinese Journal of Organ Transplantation

摘　　要：目的观察钙离子阻滞剂维拉帕米对心脏异位移植术后冠状血管内膜的影响。方法选用健康成年SD大鼠160只,随机平均分为对照组、实验1组、实验2组和实验3组。建立大鼠同种腹腔异位心脏移植模型。从移植当日到术后第9d,所有大鼠均接受环孢素A治疗,按5mg·kg-1·d-1腹腔注射,共10d;实验1、2、3组术后当天起加用维拉帕米,其剂量分别为0.1mg/kg、0.5mg/kg、1.0mg/kg,均为2次/d,连用3个月。移植后60d和90d切取移植心脏,将每例心尖组织进行HE和弹力纤维染色,根据移植心脏心尖部冠状动脉平均内膜增厚和管腔狭窄程度判定心脏移植物血管病变。结果对照组冠状血管狭窄程度较实验组明显加重,组间比较,差异有统计学意义。结论维拉帕米对大鼠移植心脏冠状血管内膜增厚有明显抑制作用。Objective To investigate the effect of calcium channel antagonist verapamil upon coronary vessel disease of allograft heart transplant rat model. Methods 160 SD rats weighing 220 to 300 g were randomly allocated to control group （group 1, n=40） and experiment groups （group 2, n = 40; group 3, n = 40, group 4, n = 40）. The hearts of all donor rats were implanted into the recipient rats. From the day after operation, the rats in each group were fed routinely and injected intraperitoneally （ip） with Cyclosporin （5 mg-kg^-1·d^-1 for 10 days）. In experiment groups, the rats were in- dividually injected （ip） with verapamil by 0.1 mg/kg, 0.5mg/kg, 1.0mg/kg twice every day for 3 months, but the rats in control group were not subjected to injection. At 60th and 90th day after grafting, 10 of each group were selected randomly and transplanted hearts were cut. The specimens were stained with HE and histochemistry. Result The degree of intimal hyperplasia in the control group was more severe than in the experiment groups. Conclusion Calcium channel antagonist verapamil can effectively inhibit the intimal hyperplasia of coronary arteries in heart transplant model.

关 键 词：冠状血管 心脏移植 维拉帕米 

分 类 号：R654.2[医药卫生—外科学]